The path followed by species in the colonization of remote oceanic islands ultimately depends on their phylogenetic constraints and ecological responses. In this study, we aim to evaluate the relative role of geographical and ecological forces in the origin and evolution of the Madeiran ivy (Hedera maderensis), a single-species endemic belonging to the western polyploid clade of Hedera. To determine the phylogenetic placement of H. maderensis within the western polyploid clade, we analyzed 40 populations (92 individuals) using genotyping-by-sequencing and including Hedera helix as outgroup. Climatic niche differences among the study species were evaluated using a database with 867 records representing the entire species ranges. To test species responses to climate, 13 vegetative and reproductive functional traits were examined for 70 populations (335 individuals). Phylogenomic results revealed a nested pattern with H. maderensis embedded within the south-western Iberian H. iberica. Gradual niche differentiation from the coldest and most continental populations of H. iberica to the warm and stable coastal population sister to H. maderensis parallels the geographical pattern observed in the phylogeny. Similarity in functional traits is observed for H. maderensis and H. iberica. The two species show leaves with higher specific leaf area (SLA), lower leaf dry matter content (LDMC) and thickness and fruits with lower pulp fraction than the other western polyploid species H. hibernica. Acquisition of a Macaronesian climatic niche and the associated functional syndrome in mainland European ivies (leaves with high SLA, and low LDMC and thickness, and fruits with less pulp content) was a key step in the colonization of Madeira by the H. iberica/H. maderensis lineage, which points to climatic pre-adaptation as key in the success of island colonization (dispersal and establishment). Once in Madeira, budding speciation was driven by geographical isolation, while ecological processes are regarded as secondary forces with a putative impact in the lack of further in situ diversification.

Background and aims: The way plants cope with biotic and abiotic selective pressures determines their success in the colonization of remote oceanic islands, which ultimately depends on the phylogenetic constrains and ecological response of the lineage. In this study we aim to evaluate the relative role of geographical and ecological forces in the origin and evolution of the Madeiran ivy (H. maderensis). Methods: To determine the phylogenetic placement of H. maderensis within the western polyploid clade of Hedera (three species), we analysed 40 populations (92 individuals) using genotyping-by-sequencing and including H. helix as outgroup. Climatic niche differences among the four study species were evaluated using a database with 706 records representing the entire species ranges. To test species responses to climate, a set of 19 vegetative and regenerative functional traits were examined for 70 populations (335 individuals). Key results: Phylogenomic results revealed a nested pattern with H. maderensis embedded within H. iberica. Gradual niche differentiation from the coldest and most continental populations of H. iberica to the warm and stable coastal population sister to H. maderensis parallels the geographical pattern observed in the phylogeny. Similarity in adaptive traits is observed for H. maderensis and H. iberica. The two species show leaves with higher SLA, lower LDMC and thickness and smaller fruits than those of H. hibernica. Conclusions: Acquisition of the Macaronesian climatic niche and the associated functional syndrome in mainland European ivies (small fruits, leaves high SLA, and low LDMD and thickness) was a key step in the colonization of Madeira 1 by the H. iberica/H. maderensis lineage, which points to climatic pre-adaptation as a driver of island colonization (dispersal and establishment). Once in Madeira, speciation was driven by geographical isolation, while ecological processes are regarded as secondary forces with a putative impact in the lack of further in situ diversification.

Premise: There has been a great increase in using climatic data in phylogenetic studies over the past decades. However, compiling the high-quality spatial data needed to perform accurate climatic reconstructions is time-consuming and can result in poor geographical coverage. Therefore, researchers often resort to qualitative approximations. Our aim was to evaluate the climatic characterization of the genera of the Asian Palmate Group (AsPG) of Araliaceae as an exemplar lineage of plants showing tropical-temperate transitions. Methods: We compiled a curated worldwide spatial database of the AsPG genera and created five raster layers representing bioclimatic regionalizations of the world. Then, we crossed the database with the layers to climatically characterize the AsPG genera. Results: We found large disagreement in the climatic characterization of genera among regionalizations and little support for the climatic nature of the tropical-temperate distribution of the AsPG. Both results are attributed to the complexity of delimiting tropical, subtropical, and temperate climates in the world and to the distribution of the study group in regions with transitional climatic conditions. Conclusions: The complexity in the climatic classification of this example of the tropical-temperate transitions calls for a general climatic revision of other tropical-temperate lineages. In fact, we argue that, to properly evaluate tropical-temperate transitions across the tree of life, we cannot ignore the complexity of distribution ranges.

PREMISE The use of climatic data on phylogenetic studies has greatly increased in the last decades. High-quality spatial data and accurate climatic information are essential to minimize errors in the climatic reconstructions to the past. However, despite the huge amount of already available biodiversity digital information, the process of compiling, cleaning, and comparing spatial data from different open data sources is a time-consuming task that sometimes ends up with low-quality geographical information. For this reason, researchers often resort qualitative approximations among which World bioclimatic classification systems or the experts' criteria are the most used. Our aim is to evaluate the climatic characterization of the genera of the Asian Palmate Group (AsPG) of the ginseng family (Araliaceae), one of the classical examples of tropical-temperate plant families. METHODS We compiled a curated worldwide spatial database of the AsPG genera. We then created five raster layers representing bioclimatic regionalizations of the World. Finally, we crossed the database with the layers to characterize the AsPG genera. RESULTS We found large disagreement in the climatic characterization of genera among regionalizations and little support for the tropical-temperate dichotomy. Both results are attributed to the complexity of delimiting tropical, subtropical and temperate climates in the World and to the distribution of the study group in regions with transitional climatic conditions. CONCLUSIONS The complexity in the climatic classification of this classical example tropical-temperate dichotomy, calls for a general revision in other families. In fact, we claim that to properly evaluate tropical-temperate transitions we cannot ignore the complexity of distribution ranges.