Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.

Cystathionine ␥-synthase, the first committed enzyme of methionine biosynthesis in higher plants, is encoded by the CGS1 gene in Arabidopsis thaliana. We have shown previously that the stability of the CGS1 mRNA is negatively regulated in response to methionine application [Chiba, Y., Ishikawa, M., Kijima, F., Tyson, R. H., Kim, J., Yamamoto, A., Nambara, E., Leustek, T., Wallsgrove, R. M. & Naito, S. (1999) Science 286, 1371-1374]. To determine whether methionine itself is the effector of the CGS1 exon 1-mediated posttranscriptional regulation, we carried out transfection experiments. The results suggested that, rather than methionine, Sadenosyl-L-methionine (AdoMet), or one of its metabolites, acts as the effector of this regulation. To further identify the actual effector, we exploited the wheat germ in vitro translation system. The effects of various metabolites and analogs of AdoMet were tested by using RNA carrying a CGS1 exon 1-reporter fusion. These tests identified AdoMet as the effector of this regulation. Sadenosyl-L-ethionine, an analog of AdoMet, also had effector activity. A. thaliana mto1 mutants, which are deficient in this regulation, showed a much reduced response to AdoMet in vitro, with a leaky allele showing a less reduced response. RNA translated in vitro in the presence of AdoMet contained a 5-truncated RNA species, similar to the one that we previously suggested was an in vivo degradation intermediate of CGS1 mRNA. Together, the results show that the basic reactions of CGS1 exon 1-mediated posttranscriptional regulation occur in the wheat germ in vitro translation system, and that AdoMet acts as the effector. C ystathionine ␥-synthase (CGS; EC 4.2.99.9) catalyzes the first committed step of methionine biosynthesis in higher plants (1) (Fig. 1), which is considered to be the key regulatory step in methionine biosynthesis (2-5). Unlike many of the key-step enzymes in metabolic pathways, CGS is not an allosteric enzyme (2). CGS is encoded in Arabidopsis thaliana by the CGS1 gene (gene ID At3g01120, GenBank accession no. AB010888) (6, 7). We have previously shown (8) that CGS1 expression involves feedback regulation at the level of mRNA stability in response to methionine application in vivo. A. thaliana mto1 mutants are deficient in this feedback regulation, and overaccumulate CGS1 mRNA, CGS protein, and soluble methionine. Seven independently isolated mto1 mutants were found to carry single-base changes within the first exon of CGS1, giving rise to amino acid sequence changes (8, 9).Transient and transgenic expression experiments using CGS1 exon 1-reporter fusions (8, 10) showed that the exon 1 coding sequence of CGS1 is necessary and sufficient for its posttranscriptional regulation, in response to exogenous application of methionine. In vitro mutagenesis of CGS1 exon 1 revealed that it is its amino acid sequence that has a role in this regulation. We have identified a stretch of 11-13 amino acid residues, termed the MTO1 region, located Ϸ80 residues from the N terminus of CGS, and coverin...

A neuroendocrine correlate of interpersonal trust is relatively unknown. We investigated the relationship between an interpersonal trust-related personality (General Trust Scale) and cortisol elevation induced by social stress in 20 men. Spearman's rank order correlation analysis revealed a significant negative correlation between social stress-induced cortisol elevation and General Trust Scale. The present results indicate subjects with higher degrees of interpersonal trust have lower levels of neuroendocrine response to social stress.

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Hepatitis E is rare in Japan but is occurring more frequently than previously thought. To investigate whether de novo subclinical infection of hepatitis E virus (HEV) has recently increased in Japan, HEV RNA was assayed in serum samples obtained from 4019 Japanese voluntary blood donors with alanine aminotransferase (ALT) of > or =61 IU/l, who are likely to have ongoing HEV infection, during 1991-2006. The overall rates of IgG-class antibody to HEV (anti-HEV IgG), anti-HEV IgM/IgA and HEV RNA among 3185 donors in 2004-2006 were comparable with those among 594 donors in 1998 (5.3 vs. 5.2%, 0.2 vs. 0.5%, and 0.2 vs. 0.3%, respectively). Among blood donors with ALT > or = 201 IU/l in three groups according to the year of blood collection (1991-1995 [n = 156], 1996-1999 [n = 116] and 2004-2006 [n = 61]), there were no appreciable differences in the prevalence of anti-HEV IgG (5.8, 4.3, and 6.6%, respectively), anti-HEV IgM/IgA (1.9, 3.4, and 3.3%, respectively) and HEV RNA (1.3, 3.4, and 3.3%, respectively). The eleven HEV isolates obtained in the present study differed from each other by 1.7-22.8% in the ORF2 sequence and segregated into genotype 3 or 4. The occurrence rate of subclinical infection with divergent HEV strains has essentially remained unchanged during 1991-2006 in Japan.