Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients
Introdução: Ainda, não existe método ideal para o diagnóstico diferencial entre estenoses biliares malignas e benignas. Este estudo visa a comparar a concentração de fibronectina biliar nos pacientes com e sem estenose biliar maligna. Métodos: Durante a pancreatocolangiografia retrógrada endoscópica (PCRE), foram coletadas amostras de bile de 50 pacientes com estenose biliar extra-hepática maligna (40), benigna (10) e de 10 doentes sem estenose biliar (grupo controle). A fibronectina total na bile foi determinada por ensaio imunoenzimático. A concentração sérica de bilirrubina direta, fosfatase alcalina, gama glutamiltransferase, aspartato aminotransferase e alanina aminotransferase foi determinada nos pacientes com estenose biliar antes da PCRE. O diagnóstico final foi definido por cirurgia, biópsia ou acompanhamento clínico. Resultados: Os pacientes com neoplasia maligna eram significativamente mais idosos (p= 0,02) e apresentaram níveis mais elevados dos testes bioquímicos relacionados à colestase (p< 0,01). Não houve diferença significativa na concentração de fibronectina biliar entre os pacientes com estenose maligna (694,2 ± 823,5 ng/ml), benigna (828,9 ± 925ng/ml) e grupo controle (466,5 ± 621,5 ng/ml), ou entre os pacientes com (721,2 ± 836,6 ng/ml) e sem estenose (466,5 ± 621,5 ng/ml). Conclusões: As médias de idade e de resultados de exames laboratoriais relacionados à colestase foram mais elevadas nos pacientes com neoplasia maligna. A dosagem apenas da fibronectina total na bile não foi eficaz para diagnóstico diferencial das estenoses biliares.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.