ResumoA ativação farmacológica dos receptores 5-HT 2C induz comportamentos de defesa em modelos animais. O estudo busca investigar se o bloqueio seletivo de receptores 5-HT 2C no hipocampo ventral (HV) previne comportamentos defensivos induzidos por um agonista de receptor 5-HT 2C administrado perifericamente em ratos expostos ao labirinto em cruz elevado (LCE). Quinze minutos após injeções intraperitoniais (IP, 1ml/ kg) do agonista 5-HT 2C WAY-161503, ratos foram microinjetados bilateralmente no HV com o antagonista seletivo de receptores 5-HT 2C SB-242084 (0, 0,1, 0,5 ou 1.5μg). Dez minutos após, cada animal foi exposto ao LCE para o registro de categorias de ansiedade. Injeções sistêmicas do WAY-161503 reduziram seletivamente as explorações nos braços abertos e aumentaram padrões de avaliação de risco. Esse efeito foi atenuado de maneira dose-dependente pela microinjeção de SB-242084 no HV, confirmando a ação ansiogênica de agonistas 5-HT 2C e sugerindo que esse perfil comportamental seja mediado, pelo menos em parte, por receptores 5-HT 2C do HV.Palavras-chave: receptor 5-HT 2C ; hipocampo ventral; labirinto em cruz elevado. Abstract Involvement of ventral hippocampus 5-HT 2C receptors on defensive behaviors of rats in the elevated plusmaze.Pharmacological 5-HT 2C receptor activation induces defensive behaviors in several animal models of anxiety. The present study investigated whether the selective blockade of 5-HT 2C receptors in the ventral hippocampus (VH) prevents defensive behaviors induced by a 5-HT 2C agonist administered systemically in rats exposed to the elevated plus-maze (EPM). Fifteen minutes after intraperitonial (IP, 1ml/kg) injections of the selective 5-HT 2C receptor agonist WAY-161503 (3 mg/kg), rats were bilaterally microinjected with the selective 5-HT 2C antagonist SB-242084 (0, 0.1, 0.5 or 1.5μg) into the VH. Ten minutes after, each animal was exposed to the EPM for measuring classical and ethological anxiety measures. IP WAY-161503 injections selectively decreased open-arm exploration while increasing risk-assessment. This anxiogenic-like action was dose-dependently attenuated by intra-VH SB-242084 microinjections. These results not only further confirm the anxiogenic-like action of 5-HT 2C agonists, but also suggest that this behavioral profile might be mediated at least in part by VH 5-HT 2C receptors.
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