Natural membrane vesicles (n-MVs) produced by Pseudomonas aeruginosa PAO1 and PAO1 carrying plasmid pAK1900 (p-MVs) were purified and analysed for DNA content. The MVs were isolated by a procedure designed to ensure no cellular contamination from the parent MV-producing cells. Fluorometry analysis revealed that p-MVs were associated with 7?80 ng DNA (20 mg MV protein)

The in vitro activity of telavancin was tested against 743 predominantly antimicrobial-resistant, gram-positive isolates. Telavancin was highly active against methicillin-resistant staphylococci (MIC 90 , 0.5 to 1 g/ml), streptococci (all MICs, <0.12 g/ml), and VanB-type enterococci (all MICs, <2 g/ml). Time-kill studies demonstrated the potent bactericidal activity of telavancin.

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