Trastuzumab is a humanized monoclonal antibody approved for treatment of HER2 positive breast cancer. Conjugation of trastuzumab offers a promising strategy of selective anticancer therapy. Trastuzumab-emtansin is a new generation of cytotoxic drug conjugated antibody with higher tumor selectivity and less toxicity of emtansin. Conjugates of trastuzumab with bifunctional chelators (BFCs) for further radiolabeling are a step ahead in the field of radiopharmacy for therapy and imaging of aggressive HER2 positive cancers. The purpose of this study is characterization of integrity and secondary structure of antibody in already formulated lyophilized conjugates with p-SCN-Bn-DTPA-and p-SCN-Bn-1B4M-DTPA-by applying SDS-PAGE electrophoresis and Raman spectroscopy. The results are positive and give an opportunity for further radiolabeling of freeze dried conjugates.
Radioimmunoconjugates are promising agents in diagnostics and treatment of different types of cancers. The aim of this study was the formulation of stable freeze dried kits of trastuzumab with three types of bifunctional chelators for further radiolabeling. The integrity of the antibody in formulated conjugates was examinated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Trastuzumab is a humanized monoclonal antibody used in the therapy of aggressive HER2 positive breast cancer. Conjugation of trastuzumab was made with various chelators: p
Infrared and Raman spectroscopy are effective techniques that allow collecting information about secondary structure of proteins, including antibodies. Trastuzumab, antibody used in our study was in a freeze-dried form, conjugated with different bifunctional chelators and linked with the stable isotopes of lutetium and yttrium. The characterization of the final immunoconjugates showed no significant changes in the structure demonstrated by the presence of the amide bands characteristic for a α-helices and β-sheets structures. These methods could be applied during the production of the antibody freeze-dried kit formulations for the labeling with the radioactive isotopes.
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