Methods of in vitro conservation offer a number of advantages for endangered species preservation. Crambe tataria Sebeók biochemistry study (fatty acid (FA) composition, antioxidant activity (AOA), polyfructan and total soluble protein content) is fairly importaint and could show the potential value of this species in agriculture, Food and Chemical Industry or pharmacology including its use as a source of valuble genetic material and could lead to new promising sources of biofuel discovery. Also, comparison of in vitro and in vivo cultured plants could point out to the effect of in vitro culture methods on plants biochemical composition Fatty acid (FA) content was determined using Gas chromatography-mass spectrophotometry (GC/MS) of fatty acid ethers. Antioxidant activity was determined using DPPH (2,2-diphenyl-1picrylhydrazyl) radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under in vitro and in vivo conditions and seeds were used in this research. Obtained data showed that C. tataria plants had high AOA and total soluble protein content along with high total FA content along with high content of α-Linolenic acid and absence of erucic acid. Difference in biochemical composition between plants grown in aseptic and not aseptic conditions was shown.

We tested germination of seeds of Partheno-cissus quinquefolia (L.) Planch. (Vitaceae) in laboratory in response to different ecological factors: light intensity, temperature, humidity, acidity and salinity. Our results show that the most critical ecological factor is humidity. The highest rank of germination was 73 % in condition of sufficient moisture, 37 % in condition of excessive moisture and only 3 % in condition with insufficient moisture. Among variants with different reaction of medium the most successful germination was at pH 7 (73 %) and pH 9 (44 %). Another significant factor for seedlings germination of P. quinquefolia is lighting, since at full-dark, dark and semi-dark conditions the level of germination was 0 %, 2 % and 24 % respectively. The percentage of P. quinquefolia germination decreases with increasing salinity level. The most optimal germination regime was at +25 °C. Further study of P. quinquefolia biology will allow to clarify the difference in naturalization level between this species and closely related P. vitacea as well as to estimate the possibility of transformation of the introduced species into invasive.

The aim of the work was to determine the main groups of polyphenolic compounds in Quercus rubra L. raw materials. Materials and methods. The leaves and annual shoots of Northern Red Oak used for research were collected in August in Lisnyky village of Obuhiv district of Kyiv Oblast (Ukraine). Determination of the component composition and quantitative content of flavonoids (including separately catechins), hydroxycinnamic acids, and phenolic acids were carried out in the samples of air-shade-dried crushed raw material to a particle size of 3 mm by the method of high-performance liquid chromatography (HPLC). Agilent Technologies 1200 liquid chromatograph was used for liquid chromatography.Results. Using the HPLC method, 18 polyphenolic compounds were identified in leaves and annual shoots of Northern Red Oak, in particular, flavonoids: rutin, quercetin-3-β-glucoside, luteolin, neohesperidin; catechins: catechin, epicatechin, epicatechin gallate, gallocatechin; hydroxycinnamic acids: chlorogenic, caffeic, trans-ferulic, trans-cinnamic, p-coumaric, hydroxyphenylacetic, benzoic, syringic, sinapic acids; phenolic acid is gallic acid. The dominant component among flavonoids is rutin (323.43 mg/100 g) (in the composition of catechins, epicatechin gallate (25.45 mg/100 g) prevails); among hydroxycinnamic acids in Northern Red Oak raw materials, chlorogenic acid (139.62 mg/100 g) and sinapic acid (74.64 mg/100 g) prevail.Conclusions. The obtained results point to the prospects of further phytochemical and pharmacological studies of Quercus rubra raw materials, with the aim of creating new plant substances based on it with antioxidant, anti-inflammatory, and antiviral activity

In Ukraine, Allium ursinum is a rare species of the state level of conservation, isolated locations of which occur very rarely on the territory of the left bank of the river Dnipro. A new location discovered on the territory of National Nature Park “Pyriatynskyi” is isolated and confined to the floodplain gallery forest of the association Alnion incanae Pawłowski et al. 1928. On the territory of the Nature Park “Pyriatynskyi”, the species is represented by a small, incomplete population with a left-sided spectrum. Considering the chorology of the species in the region and the demographic characteristics of the population, this new locality was most likely formed as a result of the recent accidental introduction of the species from the northern enclaves of the Chernihiv oblast or purposeful planting of plants by the local citizens. Taking into account the proximity of A. ursinum growing locality to popular recreation areas, it is necessary to introduce monitoring research of the state and structure of the population and develop special protection measures to conserve this species on the territory of National Nature Park “Pyriatynskyi”.

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National Nature Park “Pyryatynsky” is a valuable reserve of flora and vegetation of the Left-Bank Dnipro. The extensive hydrological network of the Udai River and the wide representation within this nature reserve of floodplain reservoirs are the reason for the high diversity of plant communities of higher aquatic vegetation. In this article, we classified the communities of alliance Nymphaeion albae Oberdorfer 1957 in the National nature park "Pyryatynskyi" and identify the features of their syntaxonomic and ecological differentiation. The literature on this type of vegetation is fragmentary, without any geobotanical information and detailed characteristics of the structure of phytoceonoses, synecology and synchorology. All obtained results are based on original field data. In total, 46 geobotanical relieves were performed during the period 2010–2017. The description of communities was carried out within their natural boundaries. Treatment of fitosociological data was performed with the JUICE software package. The nomenclature of syntaxons was consistent with the International Code of Phytosociological Nomenclature (ICFN).The alliance Nymphaeion albae Oberdorfer 1957 on the territory of NPP "Pyriatynsky" is represented by 4 associations Nymphaeo albae–Nupharetum luteae Nowinski 1927, Nymphaeetum albae Vollmar 1947, Nymphaeetum candidae Miljan 1958 and Potamo natantis–Persicarietum natantis Knapp et Stoffers 1962 nom. mutat. propos.This is first prodrome of alliance Nymphaeion albae Oberdorfer 1957 for the territory of National Nature Park “Pyryatynskyi”. The communities of this syntaxon occupy small areas and have a limited distribution in the region. Most of them are rare and vulnerable to changes in environmental conditions. Monitoring of their structure, chorology, and dynamics is an important task to maintain and preserve the species and coenotic diversity of NPP "Pyryatynsky".

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The results of a comparative structural analysis of the annual wetland herb vegetation syntaxa (class Isoëto-Nano-Juncetea) of Pyriatynskyi National Nature Park (Poltava Oblast, Ukraine) are presented. The systematic, biomorphological, ecological and geographical structures of syntaxa were studied using cluster, discriminant and factor analysis. The principal conformity of the floristic similarity dendrogram and the previously developed classification scheme were analysed. It was revealed that the leading factor of coenosis development is the soil moisture, while most parameters of these ecotopes are constant. The critical differentiation of Nano-Cyperion and Eleocharition ovatae alliances and the legitimacy of the recognition of the Radiolion linoidis alliance and Polygono recti-Juncetum juzepczukii association as separate syntaxa of the main ranks are emphasized.