The initiation of intracellular host cell colonization by symbiotic rhizobia in Medicago truncatula requires repolarization of root hairs, which includes the rearrangement of cytoskeletal filaments. The molecular players governing microtubule (MT) reorganization during rhizobial infections remain to be discovered. Here, we identified M. truncatula DEVELOPMENTALLY-REGULATED PLASMA MEMBRANE POLYPEPTIDE (DREPP), a member of the microtubule-binding DREPP/PCaP protein family and investigated its functions during rhizobial infections. We show that rhizobial colonization of drepp mutant roots as well as transgenic roots over-expressing DREPP is impaired. DREPP re-localizes into symbiosis-specific membrane nanodomains in a stimulus-dependent manner. This subcellular segregation coincides with DREPP-dependent MT fragmentation and a partial loss of the ability to reorganize the MT cytoskeleton in response to rhizobia, which might rely on an interaction between DREPP and the MT organizing protein SPIRAL2 (SPR2). Taken together, our results reveal that establishment of symbiotic associations in M. truncatula requires DREPP in order to regulate MT reorganization during initial root hair responses to rhizobia.
Cable-based timber extraction offers some advantages with regard to impacts to forest stands and soils, and can be used under a wide range of conditions. It is important not only in steep terrain, but also increasingly in flat terrain when soils have low bearing capacity. In this study, utilization data from two commonly used tower yarding systems were analyzed: a tower yarder with a mounted processor (K507) and a medium-distance tower yarder (V400). Collected data included explanatory variables, such as the proportion of hardwood timber, length of skyline, direction of yarding and dimension of harvested timber. Data were analyzed with regard to the time required for machine installation including set-up and dismantling, machine productivity and resulting production costs. Possible combinations of machines and partial working steps were evaluated. Results indicated an increasing utilization of cable crane systems in horizontal yarding direction throughout the analyzed time period. Further, more time was required to process full trees when the K507 was used, although machine productivity increased. The proportion of processed timber that was hardwood significantly influenced installation times. Results demonstrated that, if the machines had above average productivity, total costs could be reduced in flat terrain by using a cable crane instead of conducting the extraction by skidders.
The initiation of intracellular host cell colonization by symbiotic rhizobia in Medicago truncatula requires repolarization of root hairs, which includes the re-arrangement of cytoskeletal filaments. The molecular players governing microtubule (MT) re-organization during infection remain to be discovered. Here, we identified the M. truncatula DREPP protein and investigated its functions during rhizobial infections. We show that rhizobial colonization of drepp mutant roots as well as transgenic roots over-expressing DREPP is impaired. DREPP re-localizes into symbiosis-specific membrane nanodomains in a stimulus-dependent manner.This subcellular segregation coincides with DREPP-dependent MT fragmentation and a partial loss of the ability to re-organize the MT cytoskeleton in response to rhizobia, which might relay on an interaction between DREPP and MT organizing protein SPIRAL2 (SPR2). Taken together, our results reveal that establishment of symbiotic associations in M. truncatula require DREPP in order to regulate MT reorganization during initial root hair responses to rhizobia.
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