Aims: Discovering new lead compounds against cancer and bacterial infections is a crucial step to ensuring a sustainable global pipeline for new effective drugs. These study focuses on the isolation of secondary metabolites of methanol extract from the seeds of Manilkara zapota (Sapotaceae) a Cameroonian medicinal plant. Study Design: According to the literature, plants of the genus Manilkara are potential sources of antibacterial and anticancer secondary metabolites. Methodology: The air-dried and powdered seeds (1.8 kg) of M. zapota was extracted at room temperature for 72 h with a mixture of CH 2 Cl 2 /methanol (1/1). The extract was concentrated to dryness under vacuum and the residue was subjected to repeated column chromatographic separation. The structures of the isolates were established by means of spectroscopic methods.
Aims: Discovering new lead compounds against cancer and bacterial infections is a crucial step to ensuring a sustainable global pipeline for new effective drugs. This study focus on the isolation of secondary metabolites of methanol extract from the bark of Manilkara zapota (Sapotaceae) a Cameroonian medicinal plant. Study Design: According to the literature, plants of the genus Manilkara are potential sources of antibacterial and anticancer secondary metabolites. Methodology: The air-dried and powdered bark (4.0 kg) of M. zapota was extracted at room temperature for 72 h with a methanol. The extract was concentrated to dryness under vacuum and the residue was subjected to repeated column chromatographic separation. The structures of the
The Vinylogoushemiacetals (I) react with a series of silyl enol ethers such as (II), (IV), (VI), (XI), (XIV) and (XVII) to give the products shown in the scheme.
2α,3β,,12-dien-28-oic acid (1) and 3β-acetoxy-2α,19α-dihydroxy-24-norurs-4(23),12-dien-28-oic acid (2), along with 15 known compounds were isolated from the methanol extract of the twigs of Mostuea hirsuta. While 2-hydroxymethylbenzamide (13) was isolated for the first time from the natural source, compounds 3-12 are reported here for the first time from the genus Mostuea. Their structures were elucidated by means of spectroscopic analyses including 1D-and 2D-NMR spectroscopy, high-resolution mass spectrometric data as well as comparison with data from the literature. Compounds 1, 2, 4-9 and 13 were tested against bacteria, fungi and plant pathogen oomycetes by the paper disk agar diffusion assay resulting in missing to low activities corresponding with minimum inhibitory concentrations (MICs) > 1 mg mL -1 . However, the respective compounds 1, 2, 8, 9 and 13 exhibited moderate cytotoxic activity against the human Caucasian prostate adenocarcinoma cell line PC-3, with IC 50 10.6-16.5 μm compared to the standard doxorubicin with IC 50 0.9 μm.
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