The osmosensitivity of the inwardly rectifying Cl 3 current (I Clh ), expressed by primary cultured rat neocortical astrocytes long-term treated with dibutyryl cyclic AMP, was investigated in the whole-cell and perforated-patch modes. In whole-cell experiments, whereas hypotonic extracellular solution (v v = 100 mOsmol) did not cause any change in I Clh , hypertonicity produced a slowly developing, V V40% reversible decrease in current magnitude. By contrast, in perforated-patch experiments, exposure to a less hypertonic saline (v v = 50 mOsmol) depressed the current to V V50%, and hypotonicity induced a V V50% slow increase in I Clh . These differences in osmosensitivity between the two experimental modes suggest that the osmoregulation of I Clh may be mediated by complex intracellular mechanism(s), which appear(s) to be partly compromised by the dialysis of the astrocytic cytoplasm. ß
CorrigendumCorrigendum to: Osmosensitivity of an inwardly rectifying chloride current revealed by whole-cell and perforated-patch recordings in cultured rat cortical astrocytes [FEBS Letters 492 (2001) 78^83] C
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