The characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of Candida albicans biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or Punica granatum alcoholic extract to prevent C. albicans biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (10 × 2 mm) were fabricated (n = 504) and randomly divided into groups: no treatment (control—CT), fibrin biopolymer coating (FB), fibrin biopolymer with P. granatum (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with C. albicans SC5314 (1 × 107 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (α = 0.05). The FBPg and FBCh groups inhibited the growth of C. albicans biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated (p < 0.0001). The colony forming unit (CFU) assay showed that the FB group favored the C. albicans biofilm growth at 24 h and 48 h (p < 0.0001), with no differences with CT group at 72 h (p = 0.790). All groups showed an enhancement in biofilm development up to 72 h (p < 0.0001), except the FBCh group (p = 0.100). No statistical differences were found between the PMMA base materials (p > 0.050), except in the FB group (p < 0.0001). Fibrin biopolymer, albeit a scaffold for the growth of C. albicans, when combined with chlorhexidine digluconate or P. granatum, demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm.
Objective
To systematically evaluate the effect of microwave disinfection on the dimensional stability of denture base acrylic resins.
Background
Microwave disinfection has been considered as an alternative method for disinfecting complete dentures to help prevent and treat denture stomatitis. However, data on the impact of microwave disinfection on the dimensional stability of acrylic resins are still scarce.
Methods
The PubMed/Medline, SCOPUS and EMBASE databases were searched in order to assess articles published in English up to January 2021 (CRD42021212267). We included studies that have assessed the effect of microwave disinfection, on the dimensional stability of acrylic resins, comparing them with negative or positive controls.
Results
A total of seven in vitro studies were included. The qualitative synthesis demonstrated that, in general, microwave disinfection produced more distortion on the materials than do immersion in sodium hypochlorite, chloride solution, chlorhexidine, and water immersion. However, considering the dimensional stability of the specimens, microwave disinfection at 500 W for 3 minutes, and at 450 W for 5 minutes, produced similar or better outcomes than did control groups.
Conclusion
In general, microwave disinfection promotes changes in the dimensional stability of denture base acrylic resins, and should thus be used with caution. However, microwave disinfection protocols at lower power settings (500 and 450 W) and exposure times (3 and 5 minutes) produces similar or less distortion than chemical disinfection. More studies are still required in order to evaluate the clinical and long‐term implications of microwave disinfection.
An approach on the use of CAD-CAM technology for the manufacture of removable partial dentures Uma abordagem sobre o uso da tecnologia CAD-CAM para a confecção de próteses parciais removíveis BAURU 2020 MARIANA DOMINGUES PORDEUS An approach on the use of CAD-CAM technology for the manufacture of removable partial dentures Uma abordagem sobre o uso da tecnologia CAD-CAM para a confecção de próteses parciais removíveis Dissertação constituída por artigo apresentada à
The aim of this study is to evaluate the effect of two ethyl cyanoacrylate-based adhesives on the growth of Candida albicans biofilms on a heat-polymerized resin, after 7, 14, and 30 days of exposure. Ninety circular (10 × 2 mm) heat-polymerized resin specimens were equally divided into three groups: control, conventional ethyl cyanoacrylate (ECAc), and ethyl cyanoacrylate gel (ECAg). Two layers of 50 µL of each material were applied to the respective groups. C. albicans SC5314 strain was activated and standardized to 10 7 cells/mL -1 . Specimens were immersed in 1 mL of artificial saliva and deposited in 1 mL fungal suspension, washed, and immersed in 1 mL of RPMI for 7, 14, and 30 days. The medium was changed at 48-hour intervals. The final suspension was diluted (10 -1 to 10 -4 ) and deposited on Sabouraud dextrose agar for 48 h at 37 °C. After this period, the colonies were quantified using the CFU/mL calculation. Data were evaluated using oneway ANOVA and Tukey's test for post-hoc analysis (P=0.05). It was observed that both adhesives significantly reduced (P<0.05) biofilm formation compared to the control at all evaluated periods. In conclusion, an immediate and long-term inhibitory effect on C. albicans biofilm formation was observed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.