Maspin (SerpinB5) is an epithelial-specific tumor suppressor gene product that displays context-dependent cellular functions. Maspin-deficient mouse models created to date have not definitively established maspin functions critical for cancer suppression. In this study, we generated a mouse strain in which exon 4 of the Maspin gene was deleted, confirming its essential role in development but also enabling a breeding scheme to bypass embryonic lethality. Phenotypic characterization of this viable strain established that maspin deficiency was associated with a reduction in maximum body weight and a variety of context-dependent epithelial abnormalities. Specifically, maspin-deficient mice exhibited pulmonary adenocarcinoma, myoepithelial hyperplasia of the mammary gland, hyperplasia of luminal cells of dorsolateral and anterior prostate, and atrophy of luminal cells of ventral prostate and stratum spinosum of epidermis. These cancer phenotypes were accompanied by increased inflammatory stroma. These mice also displayed the autoimmune disorder alopecia aerate. Overall, our findings defined context-specific tumor suppressor roles for maspin in a clinically relevant model to study maspin functions in cancer and other pathologies.

Bilateral sequential cochlear implantation can be performed safely in patients with EVA. Audiometric outcomes are excellent and comparable to pediatric cochlear implant patients with no malformations. CSF gusher rates can be minimized by trans-round window approach. Further long-term studies are needed to identify differences within IP-EVA spectrum deformities, audiometric outcomes, and proportions of EVA patients who will need cochlear implantation for hearing rehabilitation.

Despite the promising clinical outcome, the primary challenge of the curative cancer immunotherapy is to overcome the dichotomy of the immune response: tumor-evoked immunostimulatory versus tumor-induced immunosuppressive. The goal needs to be two-fold, to re-establish sustainable antitumor-cancer immunity and to eliminate immunosuppression. The successful elimination of cancer cells by immunosurveillance requires the antigenic presentation of the tumor cells or tumor-associated antigens and the expression of immunostimulatory cytokines and chemokines by cancer and immune cells. Tumors are heterogeneous and as such, some of the tumor cells are thought to have stem cell characteristics that enable them to suppress or desensitize the host immunity due to acquired epigenetic changes. A central mechanism underlying tumor epigenetic instability is the increased histone deacetylase (HDAC)-mediated repression of HDAC-target genes regulating homeostasis and differentiation. It was noted that pharmacological HDAC inhibitors are not effective in eliminating tumor cells partly because they may induce immunosuppression. We have shown that epithelial-specific tumor suppressor maspin, an ovalbumin-like non-inhibitory serine protease inhibitor, reprograms tumor cells toward better differentiated phenotypes by inhibiting HDAC1. Recently, we uncovered a novel function of maspin in directing host immunity towards tumor elimination. In this review, we discuss the maspin and maspin/HDAC1 interplay in tumor biology and immunology. We propose that maspin based therapies may eradicate cancer.

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<div>Abstract<p>Maspin (SerpinB5) is an epithelial-specific tumor suppressor gene product that displays context-dependent cellular functions. Maspin-deficient mouse models created to date have not definitively established maspin functions critical for cancer suppression. In this study, we generated a mouse strain in which exon 4 of the <i>Maspin</i> gene was deleted, confirming its essential role in development but also enabling a breeding scheme to bypass embryonic lethality. Phenotypic characterization of this viable strain established that maspin deficiency was associated with a reduction in maximum body weight and a variety of context-dependent epithelial abnormalities. Specifically, maspin-deficient mice exhibited pulmonary adenocarcinoma, myoepithelial hyperplasia of the mammary gland, hyperplasia of luminal cells of dorsolateral and anterior prostate, and atrophy of luminal cells of ventral prostate and stratum spinosum of epidermis. These cancer phenotypes were accompanied by increased inflammatory stroma. These mice also displayed the autoimmune disorder alopecia aerate. Overall, our findings defined context-specific tumor suppressor roles for maspin in a clinically relevant model to study maspin functions in cancer and other pathologies. <i>Cancer Res; 77(4); 886–96. ©2017 AACR</i>.</p></div>

<p>List of supplemental materials accompanying the current manuscript: 1. Table S1. List of primers and expected amplicon sizes for genotyping and mRNA detection. 2. Table S2. Live births & gender distribution. 3. Figure S1. Tertiary maspin structure. 4. Figure S2. qRT-PCR detection of mRNA in mouse mammary gland and small intestine tissues using primers PB1687/1688. 5. Figure S3. Identification of PrPC mRNA. 6. Figure S4. Histopathological (H&E) and immunohistological (IHC) analysis of mouse tissues. 7. Figure S5. Mouse growth analysis. 8. Figure S6. Histopathological analysis of mouse testes and ovaries. 9. Figure S7. Mammary gland adenoma of mouse KO tissue. 10. Figure S8. Histomorphometry of wt/wt mouse prostate. 11. Figure S9. Histopathological analysis of mouse small intestine tissues.</p>

<p>List of supplemental materials accompanying the current manuscript: 1. Table S1. List of primers and expected amplicon sizes for genotyping and mRNA detection. 2. Table S2. Live births & gender distribution. 3. Figure S1. Tertiary maspin structure. 4. Figure S2. qRT-PCR detection of mRNA in mouse mammary gland and small intestine tissues using primers PB1687/1688. 5. Figure S3. Identification of PrPC mRNA. 6. Figure S4. Histopathological (H&E) and immunohistological (IHC) analysis of mouse tissues. 7. Figure S5. Mouse growth analysis. 8. Figure S6. Histopathological analysis of mouse testes and ovaries. 9. Figure S7. Mammary gland adenoma of mouse KO tissue. 10. Figure S8. Histomorphometry of wt/wt mouse prostate. 11. Figure S9. Histopathological analysis of mouse small intestine tissues.</p>