Photonic crystal fiber (PCF) is employed as a refractive index sensor (RIS) for solving a lot of problems in biological, physicochemical, medical, engineering fields and many environmental challenges, where it is used in many industries of food, medicines, chemical materials and material diagnosis. The kind of the PCFs was HC-1550 with three wavelengths of laser source were used; 638 nm, 850 nm, and 1550 nm that are useful for recording the intended transmitted signal intensity. Therefore, the measured RI was in the range (1.469 -1.455 RIU) at the temperature range (36 -70)˚C for the EBBA and it was (1.621 -1.612 RIU) at the temperature range (22 -42)˚C for the MBBA. The results showed that the highest RI sensitivity was 96.335 dBm/RIU for the HC-1550 infiltrated with MBBA using the laser of wavelength 638 nm, Also, the highest temperature sensitivity was 0.2505 dBm/˚C for empty HC-1550 using the laser of wavelength 1550 nm.
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