Type 2 diabetes mellitus (T2DM) is strongly associated with obesity. The adipose tissue secretes bioactive adipokines leading to low grade inflammation, amplified by oxidative stress, which promotes the formation of advanced glycation end products and eventually leads to dyslipidemia and vascular complications. The aim of this study was to correlate anthropometric, biochemical and oxidative stress parameters in newly diagnosed (ND) T2DM patients and to investigate the role of oxidative stress in T2DM associated with obesity. A group of 115 ND- T2DM patients was compared to a group of 32 healthy subjects in terms of clinical, anthropometric, biochemical and oxidative stress parameters. ND-T2DM patients had significantly lower adiponectin, glutathione (GSH) and gluthatione peroxidase (GPx) and elevated insulin, proinsulin, HOMA-IR index, proinsulin/insulin (P/I) and proinsulin/adiponectin (P/A) ratio, fructosamine, and total oxidant status (TOS). The total body fat mass was positively correlated with total oxidant status (TOS). Positive correlations were found between TOS and glycated hemoglobin (HbA1c), and between TOS and glycaemia. Negative correlations were identified between: GPx and glycaemia, GPx and HbA1c, and also between GSH and fructosamine. The total antioxidant status was negatively correlated with the respiratory burst. The identified correlations suggest the existence of a complex interplay between diabetes, obesity and oxidative stress.

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547.913 and M. V. Bubulica 1* Sambucus ebulus L. is a common species of Eurasian origin that is mentioned as belonging to flora of different countries >1-3@.Carl von Linne is the first to give authenticity to the genus and its names and species while providing a detailed and complete description of them [4].From Sambucus ebulus L. are harvested rhizomes, roots, leaves, flowers, and fruits. Sambucus ebulus L. is known in Romanian traditional medicine mainly for its bacteriostatic and diuretic action. It is harvested from June to August [5].Plants were collected from the village of Imnicu de Sus, Dolj, Romania during July-August 2008. After harvesting, the leaves were dried at temperatures between 20-25qC in a dark room. After drying, the leaves were ground and extracted with ethyl alcohol and ethyl ether.The mass of the extraction cartridge was 10 to 15 g of spray product. Extraction with diethyl ether lasted 8 hours in a Soxhlet extractor. After cooling, the system was washed with ether, and the combined extracts were concentrated by distillation in a rotavapor to 50 mL volume and analyzed by GC-MS. The cartridge with residues was dried and weighed.Examined material: Sambucus ebulus L. leaves; substances: solvents (ethyl alcohol, ethyl ether); equipment: Hewlett Packard 6890 gas chromatograph with 5973 mass spectrometer detector, column: BD1 30 m u 0.25 mm u 1 Pm, T inj .: 290qC, carrier gas flow rate (He) = 0.8 mL/min, split ratio 188:1, = 188:1, temperature source MS = 230qC, quadrupole MS = 150qC, interface MS = 300qC, Soxhlet extractor, LABOROTA rotavapor 4000.Results of extraction: 2.156% extractable compounds in ether and 11.43% extractable compounds in alcohol.Test results are presented in Tables 1 and 2. A large number of compounds were identified in Sambucus ebulus L. for the first time in the the specialty literature using a simple and inexpensive method with reduced solvent consumption. The main constituents with therapeutic properties of this plant are phenolic compounds, found in great numbers in the leaves of Sambucus, which are associated with organoleptic, nutritional, and antioxidant properties. Four of them (4-ethylcatechol, 2-methoxy-6-(2-propyl)-phenol, 4-ethyl-1,3-benzenediol, 1,2-benzenediol, 2-methoxy-4-vinylphenol) are common in medicinal plants (Maticaria chamomilla, Melilotus officinalis, L. album) and are responsible for the antiinflammatory effect of this plant [6]. The presence of a large number of hydrocarbons and ethers in plants can be correlated with their analgesic properties. Also, it is known that ethers in the composition of identified plants in traditional medicine are responsible for their antibacterial (yarrow, calendula) or calming (chamomile) properties [7]. Also, a multitude of hydrocarbons in plants can be linked with their geographical affiliation and the soil characteristics of the place where they are harvested.There have also been identified a great number of fatty acids (palmitic, linoleic, stearic, myristic, isovaleric, 3-methylvaleric acids). Isovaleric and valer...

Seven new complexes of 2-pyridyl ketone Schiff bases N,N 0 -bis-(pyridin-2-yl-benziliden)-propan-1,3diamine (bpbpd) and N,N 0 -bis-[1-(pyridin-2-yl)-etiliden]propan-1,3-diamine (bpepd) with Co(II), Ni(II), Zn(II) and Cd(II) nitrates and perchlorates were synthesized and investigated using elemental analysis, spectroscopic techniques (IR and UV-Vis-NIR) as well as magnetic and conductometric measurements. The structure of a cadmium(II) complex was accomplished by single-crystal X-ray diffraction. The Schiff base ligands act as NNNN tetradentate ligands coordinating through pyridine nitrogens and azomethine nitrogens to transition metal ions. The metal ions are six-coordinate for Co(II) and Ni(II), fourcoordinate in the case of Zn(II) and eight-coordinate for Cd(II) ions. The coordination sphere of cobalt and nickel complexes is completed by two water molecules, resulting a distorted octahedron. In the case of zinc(II) complexes has been proposed a distorted tetrahedral geometry. The Cd(II) ions are surrounded by a tetradentate Schiff base ligand and two bidentate nitrate anions, leading to a dodecahedral coordination environment. Thermal decomposition of these compounds is a multistage process. Generally, water elimination occurs in the first step and the decomposition of nitrate groups and organic ligands in the next stages. The last step of decomposition for all complexes is strong exothermic and corresponds to oxidative combustion of the Schiff base ligand.