In vivo biosensors
that can convert metabolite concentrations into
measurable output signals are valuable tools for high-throughput screening
and dynamic pathway control in the field of metabolic engineering.
Here, we present a novel biosensor in Saccharomyces
cerevisiae that is responsive to p-coumaroyl-CoA, a central precursor of many flavonoids. The sensor
is based on the transcriptional repressor CouR from Rhodopseudomonas palustris and was applied in combination
with a previously developed malonyl-CoA biosensor for dual regulation
of p-coumaroyl-CoA synthesis within the naringenin
production pathway. Using this approach, we obtained a naringenin
titer of 47.3 mg/L upon external precursor feeding, representing a
15-fold increase over the nonregulated system.
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