The Arctic is getting warmer and wetter. Here, we document two independent examples of how associated extreme precipitation patterns have severe implications for high Arctic ecosystems. The events stand out in a 23-year record of continuous observations of a wide range of ecosystem parameters and act as an early indication of conditions projected to increase in the future. In NE Greenland, August 2015, one-quarter of the average annual precipitation fell during a 9-day intensive rain event. This ranked number one for daily sums during the 1996–2018 period and caused a strong and prolonged reduction in solar radiation decreasing CO2 uptake in the order of 18–23 g C m−2, a reduction comparable to typical annual C budgets in Arctic tundra. In a different type of event, but also due to changed weather patterns, an extreme snow melt season in 2018 triggered a dramatic gully thermokarst causing rapid transformation in ecosystem functioning from consistent annual ecosystem CO2 uptake and low methane exchange to highly elevated methane release, net source of CO2, and substantial export of organic carbon downstream as riverine and coastal input. In addition to climate warming alone, more frequent occurrence of extreme weather patterns will have large implications for otherwise undisturbed tundra ecosystems including their element transport and carbon interactions with the atmosphere and ocean.
Northern permafrost soils store more than half of the global soil carbon. Frozen for at least two consecutive years, but often for millennia, permafrost temperatures have increased drastically in the last decades. The resulting thermal erosion leads not only to gradual thaw, resulting in an increase of seasonally thawing soil thickness, but also to abrupt thaw events, such as sudden collapses of the soil surface. These could affect 20% of the permafrost zone and half of its organic carbon, increasing accessibility for deeper rooting vegetation and microbial decomposition into greenhouse gases. Knowledge gaps include the impact of permafrost thaw on the soil microfauna as well as key taxa to change the microbial mineralization of ancient permafrost carbon stocks during erosion. Here, we present the first sequencing study of an abrupt permafrost erosion microbiome in Northeast Greenland, where a thermal erosion gully collapsed in the summer of 2018, leading to the thawing of 26,500-year-old permafrost material. We investigated which soil parameters (pH, soil carbon content, age and moisture, organic and mineral horizons, and permafrost layers) most significantly drove changes of taxonomic diversity and the abundance of soil microorganisms in two consecutive years of intense erosion. Sequencing of the prokaryotic 16S rRNA and fungal ITS2 gene regions at finely scaled depth increments revealed decreasing alpha diversity with depth, soil age, and pH. The most significant drivers of variation were found in the soil age, horizons, and permafrost layer for prokaryotic and fungal beta diversity. Permafrost was mainly dominated by Proteobacteria and Firmicutes, with Polaromonas identified as the most abundant taxon. Thawed permafrost samples indicated increased abundance of several copiotrophic phyla, such as Bacteroidia, suggesting alterations of carbon utilization pathways within eroding permafrost.
Advances in high-throughput sequencing (HTS) technologies and their increasing affordability have fueled environmental DNA (eDNA) metabarcoding data generation from freshwater, marine and terrestrial ecosystems. Research institutions worldwide progressively employ HTS for biodiversity assessments, new species discovery and ecological trend monitoring. Moreover, even non-scientists can now collect an eDNA sample, send it to a specialized laboratory for analysis and receive in-depth biodiversity record from a sampling site. This offers unprecedented opportunities for biodiversity assessments across wide temporal and spatial scales. The large volume of data produced by metabarcoding also enables incidental detection of species of concern, including non-indigenous and pathogenic organisms. We introduce an online app—Pest Alert Tool—for screening nuclear small subunit 18S ribosomal RNA and mitochondrial cytochrome oxidase subunit I datasets for marine non-indigenous species as well as unwanted and notifiable marine organisms in New Zealand. The output can be filtered by minimum length of the query sequence and identity match. For putative matches, a phylogenetic tree can be generated through the National Center for Biotechnology Information’s BLAST Tree View tool, allowing for additional verification of the species of concern detection. The Pest Alert Tool is publicly available at https://pest-alert-tool-prod.azurewebsites.net/.
Permafrost soils store a substantial part of the global soil carbon, but due to global warming, abrupt erosion and consecutive thaw make these carbon stocks vulnerable to microbial decomposition into greenhouse gases. Although in temperate systems trophic interactions promote soil carbon storage, their role in Arctic permafrost microbiomes, especially during thaw, remains largely unknown. Here, we investigated the microbial response to in situ thawing and rapid permafrost erosion. We sequenced the total RNA of a 1 m deep soil core from an active abrupt erosion site to analyse the microbial community in the active layer soil, recently thawed and intact permafrost, consisting of up to 26 500-year-old material. We found maximum RNA:DNA ratios in recently thawed permafrost, indicating upregulation of protein biosynthesis upon thaw. At the same depths, the relative abundance of several prokaryotic orders, including Sphingobacteriales, Burkholderiales, and Nitrosomonadales increased in relative abundance. Bacterial predators were mainly dominated by Myxococcales. Protozoa were overall less abundant but doubled in relative abundance between the active layer and recently thawed permafrost. Cercozoa, Amoebozoa, and Ciliophora were the most abundant protozoan predators, replacing myxobacteria at deeper thaw depths. Overall, connections between the active layer and especially upper thawed layers were visible and suggest migration, while no layer formed a distinct community. Our findings highlight the importance of predation and population dynamics as well as the rapid development of a microbial bloom in abruptly thawing permafrost.
<p>In recent years, permafrost-affected soils have been shown to be gradually subject of thawing (IPCC, 2019). Formerly frozen soil organic carbon stocks hence become increasingly susceptible to microbial decomposition and transformation into greenhouse gases (Schuur<em> et al</em>., 2015). An estimated 20% of Arctic permafrost areas are subject of melting of belowground ice and consequent collapse (Olefeldt <em>et al.</em> 2016), but these thermokarst landscapes are often difficult to assess.</p><p>In 2018, a thermokarst developed into a thermal erosion gully in close vicinity to the Zackenberg Research Station. As one of the main stations of the Greenland Ecosystem Monitoring (GEM) program, the monitoring of various ecosystem parameters at this site during the past 25 years, including hydrology, soil temperature and active layer depth, enables a spatiotemporally precise description of the thermokarst's physical progression.</p><p>In order to characterize the development of a thermokarst soil microbial community and understand its spatial distribution and taxonomic biodiversity, soil cores of 30 cm above and below an ice lens were extracted in August 2018, as well as after a dry and warm summer season in September 2019, until 90 cm depth to also sample still frozen permafrost soils. Soil characterization included loss on ignition, radiocarbon dating and microbial viability assays for both years. Bacterial 16S rDNA V3-V4 and fungal ITS1 gene region amplicons of extracted DNA were sequenced and analyzed. With the microbiome involved in biochemical processes such as nitrogen fixation, methane production and oxidation as well as CO<sub>2</sub> respiration, knowledge about abundance, genetic and adaptation potential of bacteria, archaea and microeukaryotes in fast changing permafrost soils affects several ecosystem carbon fluxes significantly.</p><p>This work is part of a project, describing both the taxonomic and functional composition of this thermokarst microbiome, including the use of multi-omics to reveal the carbon cycling gene potential and expression in combination with in situ and laboratory incubation gas fluxes of CO<sub>2</sub>, N<sub>2</sub>O and CH<sub>4</sub>. These biological and biogeochemical insights from this event are put into perspective with long-term, maintained data supplied by the GEM.&#160;</p>
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