By filtering relevant sensory inputs and initiating stress responses, the brain is an essential organ in stress coping and adaptation. However, exposure to chronic or repeated stress can lead to allostatic overload, where neuroendocrinal and behavioral reactions to stress become maladaptive. This work examines forebrain mechanisms involved in allostatic processes in teleost fishes. Plasma cortisol, forebrain serotonergic (5-HTergic) neurochemistry, and mRNA levels of corticotropin-releasing factor (CRF), CRF-binding protein (CRF-BP), CRF receptors (CRFR1 and CRFR2), mineralocorticoid receptor (MR), glucocorticoid receptors (GR1 and GR2) and serotonin type 1A (5-HT 1A ) receptors (5-HT 1Aα and 5-HT 1Aβ ) were investigated at 1 h before and 0, 1 and 4 h after acute stress, in two groups of rainbow trout held in densities of 25 and 140 kg m −3 for 28 days. Generally, being held at 140 kg m −3 resulted in a less pronounced cortisol response. This effect was also reflected in lower forebrain 5-HTergic turnover, but not in mRNA levels in any of the investigated genes. This lends further support to reports that allostatic load causes fish to be incapable of mounting a proper cortisol response to an acute stressor, and suggests that changes in forebrain 5-HT metabolism are involved in allostatic processes in fish. Independent of rearing densities, mRNA levels of 5-HT 1Aα and MR were downregulated 4 h post-stress compared with values 1 h post-stress, suggesting that these receptors are under feedback control and take part in the downregulation of the hypothalamic-pituitary-interrenal (HPI) axis after exposure to an acute stressor.

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Comparative models suggest that effects of dietary tryptophan (Trp) on brain serotonin (5-hydroxytryptamine; 5-HT) neurochemistry and stress responsiveness are present throughout the vertebrate lineage. Moreover, hypothalamic 5-HT seems to play a central role in control of the neuroendocrine stress axis in all vertebrates. Still, recent fish studies suggest long-term effects of dietary Trp on stress responsiveness, which are independent of hypothalamic 5-HT. Here, we investigated if dietary Trp treatment may result in long-lasting effects on stress responsiveness, including changes in plasma cortisol levels and 5-HT neurochemistry in the telencephalon and hypothalamus of Atlantic salmon. Fish were fed diets containing one, two or three times the Trp content in normal feed for 1 week. Subsequently, fish were reintroduced to control feed and were exposed to acute crowding stress for 1 h, 8 and 21 d post Trp treatment. Generally, acute crowding resulted in lower plasma cortisol levels in fish treated with 3×Trp compared with 1×Trp- and 2×Trp-treated fish. The same general pattern was reflected in telencephalic 5-HTergic turnover, for which 3×Trp-treated fish showed decreased values compared with 2×Trp-treated fish. These long-term effects on post-stress plasma cortisol levels and concomitant 5-HT turnover in the telencephalon lends further support to the fact that the extrahypothalamic control of the neuroendocrine stress response is conserved within the vertebrate lineage. Moreover, they indicate that trophic/structural effects in the brain underlie the effects of dietary Trp treatment on stress reactivity.

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In animals, personality variations in response to stress and energy demands have been established. Cognitive processing of negative stimuli correlates with stress response patterns. Still, the relative contribution of cognitive appraisal or physiological demands to the behavioural output needs to be clarified. In this study we utilized reactive (high-responsive, HR) and proactive (low-responsive, LR) rainbow trout strains to investigate how contrasting reactions to hypoxia are related to individual variation in metabolism and/or cognition. The HR-LR strains did not differ in standard metabolic rate or hypoxia tolerance. HR trout displayed more pronounced avoidance to a signal cue after being conditioned with hypoxia, suggesting that they experienced this stimulus more aversive than LR trout. Together with differences in forebrain c-fos activation patterns in dorsomedial pallium, these results suggest cognitive differences between the strains. These results demonstrate that differences in personality/stress coping style can be related to contrasts in cognition, which are independent of metabolic differences.

When foraging for food, animals must track the sensory events of their environment and their own actions over time. Memory of these sensorimotor events is crucial for learning the values of different options and foraging policies 1. To investigate the role of the medial prefrontal cortex (mPFC) in foraging behavior, we conducted experiments on mice using foraging tasks that required integration of past oro-sensory rewards and past choices. We found that the mPFC selectively represents sensorimotor events, which organize into a spatiotemporal map encoding location and temporal delay of past rewards and choices relative to the animal's current epoch in time. These representations of sensorimotor events, which we refer to as sensorimotor state representations, play a critical role in foraging behavior. Inactivation of the mPFC affected the integration of past rewards and choices into the mice's decisions, leading to a decrease in reward harvesting efficiency, particularly for longer temporal delays. Behavioral models that compute values and policy failed to capture the representations in mPFC. Our results suggest that the mPFC plays a critical role in representing sensorimotor states independently of value and policy computations. This highlights the importance of considering sensorimotor state representation in the mPFC in understanding foraging behavior.