The aim of this study was to evaluate changes in polyphenol profile and antioxidant capacity of five soluble coffees throughout a simulated gastro-intestinal digestion, including absorption through a dialysis membrane. Our results demonstrate that both polyphenol content and antioxidant capacity were characteristic for each type of studied coffee, showing a drop after dialysis. Twenty-seven compounds were identified in coffee by HPLC-MS, while only 14 of them were found after dialysis. Green+roasted coffee blend and chicory+coffee blend showed the highest and lowest content of polyphenols and antioxidant capacity before in vitro digestion and after dialysis, respectively. Canonical correlation analysis showed significant correlation between the antioxidant capacity and the polyphenol profile before digestion and after dialysis. Furthermore, boosted regression trees analysis (BRT) showed that only four polyphenol compounds (5-p-coumaroylquinic acid, quinic acid, coumaroyl tryptophan conjugated, and 5-O-caffeoylquinic acid) appear to be the most relevant to explain the antioxidant capacity after dialysis, these compounds being the most bioaccessible after dialysis. To our knowledge, this is the first report matching the antioxidant capacity of foods with the polyphenol profile by BRT, which opens an interesting method of analysis for future reports on the antioxidant capacity of foods.
Polymorphism of caprine milk proteins was studied by capillary
electrophoresis. Identification of casein (CN) fractions was effected by
using isolated
fractions from cation-exchange fast protein liquid chromatography. Genetic
polymorphisms in caprine αs2-CN, αs1-CN,
β-CN and κ-CN have been determined. κ-CN A and B,
β-CN A and null, αs2-CN A, B and C,
αs1-CN A, B, C and null, and other
forms with intermediate and low αs1-CN content have been
identified. The capillary
electrophoresis method made it possible to analyse whole caprine milk using
simple
sample preparation and was rapid, automated and suitable for phenotyping
studies.
This method may also permit the quantitative study of different protein
fractions.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.