ABSTRACT

(88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA.

Objective: Propolis is a bee product useful natural substance applied in medicine. We have investigated the effect of propolis extract on biodistribution of sodium pertechnetate (Na 99m TcO 4) and laboratorial parameters in rats. Methods: It was administered, by gavage, 1.0ml/day of propolis into 10 rats (treated group) during 10 days and 1.0 ml/day of saline solution into other 10 rats (control group), by the same via and period. On the 10 th day, after 60min, all the animals received 0.1ml of Na 99m TcO 4 by ocular via. 50% of each group of rats was killed after 10min and other 50%, after 30min. Samples of brain, stomach, small bowel, liver, kidneys, thyroid and lungs were isolated and the %ATI/g of each organ was calculated. Tissue samples were taken from all the organs and stained by HE and PAS. Data were compared by Mann-Whitney and Student's t-tests (p<0.05). Results: There was a statistically significant increase in the %ATI/g, from control to treated group, respectively, in small bowel (0.15±0.01 to 0.32±0.09, after 10min and 0.32±0.03 to 0.42±0.02, after 30min) and blood (0.22±0.01 to 0.48±0.02, after10min and 0.38±0.02 to 0.51±0.04, after 30min).Conclusion: Propolis extract facilitated the uptake of Na 99m TcO 4 in some organs of rats and increased levels of some laboratorial parameters.

Purpose: Many patients with metastatic bone disease have to use radiopharmaceuticals associated with chemotherapy to relieve bone pain. The aim of this study was to assess the influence of docetaxel on the biodistribution of samarium-153-EDTMP in bones and other organs of rats. Methods: Wistar male rats were randomly allocated into 2 groups of 6 rats each. The DS (docetaxel/samarium) group received docetaxel (15 mg/kg) intraperitoneally in two cycles 11 days apart. The S (samarium/control) group rats were not treated with docetaxel. Nine days after chemotherapy, all the rats were injected with 0.1ml of samarium-153-EDTMP via orbital plexus (25µCi). After 2 hours, the animals were killed and samples of the brain, thyroid, lung, heart, stomach, colon, liver, kidney and both femurs were removed. The percentage radioactivity of each sample (% ATI/g) was determined in an automatic gamma-counter (Wizard-1470, Perkin-Elmer, Finland). Results: On the 9 th day after the administration of the 2 nd chemotherapy cycle, the rats had a significant weight loss (314.50±22.09g) compared (p<0.5) to pre-treatment weight (353.66± 22.8). The % ATI/g in the samples of rats treated with samarium-153-EDTMP had a significant reduction in the right femur, left femur, kidney, liver and lungs of animals treated with docetaxel, compared to the control rats. Conclusion: The combination of docetaxel and samarium-153-EDTMP was associated with a lower response rate in the biodistribution of the radiopharmaceutical to targeted tissues. Further investigation into the impact of docetaxel on biodistribution of samarium-153-EDTMP would complement the findings of this study. Key words: Taxoids. Samarium. Biological Availability. Drug Therapy. Rats. RESUMOObjetivo: Muitos pacientes com metástases ósseas são tratados com radiofármacos associados com quimioterapia para alívio da dor óssea. O objetivo do trabalho foi estudar a influência do docetaxel na biodistribuição do EDTMP-153-samário nos ossos e outros órgãos de ratos. Métodos: Ratos Wistar foram aleatoriamente alocados em 2 grupos de 6 animais cada. O grupo DS (docetaxel/samário) recebeu docetaxel (15 mg/kg) intraperitoneal em dois ciclos com 11 dias de intervalo. Os ratos do grupo S (samário/controle) não foram tratados com docetaxel. Nove dias após a quimioterapia, todos os animais receberam 0,1ml de EDTMP-153-samário via plexo orbital (25µCi). Após 2 horas, os animais foram mortos e feitas biópsias de cérebro, tireóide, pulmão, coração, estômago, cólon, fígado, rim e fêmures. O percentual de radioatividade por grama (%ATI/g) de tecido de cada biópsia foi determinado em contador gama automático (Wizard-1470, Perkin-Elmer, Finland). Resultados: No 9º após 2º ciclo de docetaxel os ratos tiveram perda de peso significante, passando de 353,66± 22,8g (controle/pré-tratamento) para 314,50±22,09g (p<0,5). Os % ATI/g nos órgãos dos ratos tratados com EDTMP-153-samário e docataxel tiveram redução significante nos fêmures direito e esquerdo, rim, fígado e pulmão, quando comparados com os não trata...