The South American native species Tuta absoluta is recognized as a tomato pest with fulminant dispersal ability in the new invaded areas. T. absoluta monitoring in all tomato-producing regions of the country is a vital step in early detection and integrated management decision-making. The aim of the study was to elucidate the interval of occurrence and population dynamics of T. absoluta in Muntenia region, respectively the vegetable basin that supply Bucharest northern markets. The paper presents the results of pest monitoring carried out in 2016, 2017, 2018 and 2019, by using pheromone-baited traps and also by visual inspection of tomato crops in various greenhouses in four localities from Bucharest surrounding area. The study provides first systematic monitored data about T. absoluta dynamics in protected tomato crops from southern Romania. Depending on year weather conditions, in the greenhouses tomato crops from the investigated area, the tomato leaf miner completed maximum 3 to 4 generations from spring to autumn and the degree of attack on tomatoes reached its maximum at the end of the second vegetation cycle. There was also noted a steady annual increase in the number of adult catches and degree of attack on tomatoes in the protected crop from analysed area.
The aim of the study was to update knowledge of the complex of harmful species that damage apple tree buds, flowers and leaves in orchards in the southern part of Romania. The pest fauna identified by visual observation and collection of infested plant material in the 2021 study, performed in 3 orchards, was represented by a total of 4 species of beetles: Phyllobius pomaceus, P. oblongus, Anthonomus pomorum (Coleoptera: Curculionidae) and Tropinota (Epicometis) hirta (Coleoptera:Scarabaeidae) and 12 species of moths: Phyllonorycter blancardella (Lepidoptera: Gracillariidae), Stigmella malella (Lepidoptera:Nepticulidae), Hyphantria cunea (Lepidoptera:Erebidae), Leucoptera malifoliella, Lyonetia clerkella (Lepidoptera: Lyonetiidae), Adoxophyes orana, Archips podanus, A. rosanus, Clepsis consimilana, Pandemis heparana, P. cerasana and Hedia nubiferana (Lepidoptera:Tortricidae). The report reveals the variations among orchards, in terms of species composition, relative abundance of common species and their importance for the region.
The grey corn weevil, Tanymecus (Episomecus) dilaticollis Gyll., (Curculionidae: Entiminae) is the most destructive pest of maize and sun flower crops in Romania. In this article we report result of evaluation of native strains of entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. (Deuteromicotina: Hyphomycetes) and B. pseudobassiana Rehner et Humber against Tanymecus dilaticollis (Curculionidae: Entiminae) both in laboratory and field. In laboratory assay, two different strains of B. bassiana and one strain of B. pseudobassiana (BbLy) were applied on insects as 1×108 conidia/ml aqueous suspensions. The pure ATCC 74040 commercial strain of B. bassiana and the commercial mycoinsecticide based on this strain (Naturalis) were included in the laboratory assay for comparison. Adult mortalities were recorded daily, 14 days post-exposure. All the fungal strains have been shown to be pathogenic to T. dilaticollis. The B. bassiana strain (BbTd1) and the B. pseudobassiana strain (BbLy) were effective against adults of T. dilaticollis and comparable in percentage of mycosis and virulence to the B. bassiana strain ATCC 74040. The commercial product Naturalis was superior to the tested fungal strains killing the insects within a day. In the field, the strains BbTd1 and BbLy applied as conidia multiplied on barley grains (1×109 /g d.w) in the soil did not affect the T. dilaticollis density in maize crop.
The present study aimed at examining the efficacy of two local Beauveria strains, used separately or simultaneously with a native predatory bug species and their interactions against the larvae of Tuta absoluta on tomato plants under laboratory conditions. The fungal treatments were done with concentrations of 1 × 105, 1 x 107 and 1 x 109 conidia ml-1.. Seven days after treatment application, both Beauveria isolates induced a Tuta absoluta mortality above 80%, the best results being registered in case of combination between the use of B. pseudobassiana isolate at a concentration of 1 x 107 conidia ml-1 and the predator Arma custos. No side-effects on natural enemies used in combination indicate these isolates as promising alternative for Tuta absoluta control.
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