The present study aimed at examining the efficacy of two local Beauveria strains, used separately or simultaneously with a native predatory bug species and their interactions against the larvae of Tuta absoluta on tomato plants under laboratory conditions. The fungal treatments were done with concentrations of 1 × 105, 1 x 107 and 1 x 109 conidia ml-1.. Seven days after treatment application, both Beauveria isolates induced a Tuta absoluta mortality above 80%, the best results being registered in case of combination between the use of B. pseudobassiana isolate at a concentration of 1 x 107 conidia ml-1 and the predator Arma custos. No side-effects on natural enemies used in combination indicate these isolates as promising alternative for Tuta absoluta control.
The objective of this work was testing 4 volatile oils obtained from new varieties of medicinal plants (yellow basil; red basil; marigold; hyssop), over Beauveria brongniartii (BbgMm1a/09) entomopathogenic fungi and Sitophilus granarius, deposit insect. The results obtained showed that at different concentrations, red basil volatile oil showed the strongest inhibitory effect compared to the other tested oils (yellow basil, marigold and hyssop). Concerning the insecticidal action, the preliminary tests with the 4 volatile oils obtained from new varieties of medicinal plants had no effect on the test insect - Sitophilus granarius.
The in vitro effect of three herbicides commonly used in wheat or corn plant protection programs in Romania on mycelial growth of entomopathogenic Beauveria bassiana (BbTd1) were investigated. The formulations with Isoxaflutole 225 g/L + Thiencarbazone methyl 90 g/L + cyprosulfamide (safener) 150 g/L (Adengo), Nicosulfuron 40g/L (Nicogan) and dimethylamine salt 600g/L (Dicopur) were tested at three different concentrations (field recommendation- FR, half and twice the FR) both on solid and liquid media. On the solid media, the radial growth of the fungus was measured for 11 days. On the liquid media mycelial biomass was weighted after 7 days of incubation on a rotary shaker. Dicopur significantly inhibited the vegetative growth of B. bassiana BbTd1 in both culture media at tested concentrations. In liquid media, mycelial growth was stimulated by Adengo and Nicogan at different concentrations. No significant differences caused by Adengo and Nicogan compared to control of radial growth was detected. The results showed that Adengo and Nicogan could be used simultaneously with this entomopathogen in integrated control programs. Further in vivo studies are necessary to establish if Dicopur formulation can cause damage to field populations of entomopathogenic fungi.
The thermal tolerance of four isolates of Beauveria bassiana and one isolate of Beauveria pseudobassiana was evaluated in vitro, by measuring the colonial diameters, on PDA medium, at temperatures between 5 and 35 ° C, during 14 days. The data obtained were used to calculate the growth rate of fungal colonies (mm / day), using linear regression. The representation of the values corresponding to the minimum, optimal and maximum temperature for vegetative growth was a curve described by a modified beta (ß) mathematical function. The minimum growth temperature of these isolates varied between 3.4 and 4.5 °C, the optimum temperature varied between 21.8 and 22.9 °C, except for one isolate of which optimal temperature was 26.8 °C, while the maximum temperature was varied for all isolates between 35.0 and 35.7 °C.
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