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The main aim of this work was to provide baseline data on aspects of prooxidant and antioxidant processes in different life-stages of the marine amphipod Gammarus locusta. The activities of antioxidant enzymes and levels of lipid peroxidation were determined in whole body juveniles, sub-adults and male and female adults of a laboratory population of G. locusta. Fatty acid composition of individuals at these different stages of development was also characterised in order to examine the contribution that polyunsaturated fatty acids (PUFA) might make to the peroxidation status of animals. The antioxidant enzymes, measured in whole body 100, 000 supernatants, comprised catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and glutathione peroxidase (GPX; EC 1.11.1.9). Fatty acids were analysed as fatty acid methyl esters (FAME). Lipid peroxidation was examined in terms of the levels of lipid peroxides determined as thiobarbituric acid-reactive malondialdehyde equivalents. Age-related changes were seen in antioxidant enzyme status: levels of SOD (p< 0.01) and GPX (p< 0.001) activities decreased progressively during development from juveniles to adults. Sex-related changes in GPX activity were also seen, the levels being higher in adult males than females (p< 0.001). The amount of FAME present in whole body amphipod also changed over the life span. Among PUFA, the eicosapentaenoic (C20:5n-3), arachidonic (C20:4n-6) and docosahexaenoic acids (C22:6n-3) were the most abundant acids in this species, and both their individual concentrations and total PUFA increased progressively with age (up to 3.3-fold; p< 0.001). The latter changes may contribute to the explanation of the observed differences in peroxidation status of the animals with age; thus, levels of lipid peroxides increased up to 40 % in adult males compared to other age-classes (p< 0.001). Overall, the decline in antioxidant enzyme activities, coupled with increased levels of PUFA, as the

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Unusual difficulties are encountered when characterizing the spatial distribution of the properties that collectively define the state of estuaries. Due to the variability of these estuarine conditions, greater sampling efforts are often necessary to describe estuarine environments, as compared to other aquatic systems. That is why in coastal management studies, where the collection of data is sometimes very difficult and time-consuming, a robust sampling strategy is essential. The aim of this study is to design a spatial sampling strategy for estuarine sediments, using prior information on the spatial variation of sediment granulometry. Systematic unaligned sampling with a grid cell size of 750 × 500 m was chosen on the basis of semi-variogram analysis, and was shown to have distinct advantages. This design was sampled for sediment parameters using a GPS-receiver and mapped within the digitized shoreline of the estuary. The estuary shoreline was digitized on the basis of aerial ortho-photography with tidal ebb determination. The sampling is intended to define the boundaries of environmental management areas for the Sado Estuary, situated on the west coast of Portugal. The research represents one of the initial phases in the development of a Sado Estuary environmental management system integrated into a Geographic Information System.