ResumenSe aislaron diferentes microorganismos de varios ríos de la Huasteca Potosina en México, los cuales crecen en presencia de petróleo como única fuente de carbono. Se determinó la actividad de alcohol oxidasa por un método colorimétrico. La bacteria más frecuentemente encontrada fue Pseudomonas aeruginosa (50%) y se encontró solamente una levadura, Candida albicans (6.25%). Además, presentan buena actividad de alcohol oxidasa en la fracción citosólica con diferentes sustratos. Se concluye que estos microorganismos pueden ser utilizados para la eliminación y degradación de petróleo en sitios contaminados. Palabras clave: microorganismos, bacterias, contaminación por petróleo, Candida albicans, alcohol oxidasa Isolation and Identification of Petroleum Resistant Bacteria and Yeast AbstractDifferent microorganisms were isolated from various rivers of the Huasteca Potosina, in Mexico that grow in the presence of petroleum as the sole carbon source. The activity of alcohol oxidase was determined by a colorimetric method. The most common bacteria present in the samples were Pseudomonas aeruginosa (50%) and only one type of yeast was found, Candida albicans (6.25%). Furthermore, they show good activity of alcohol oxidase in the cytosolic fraction with different substrates. It was concluded that this microorganisms could be used for decontamination of aquatic habitats polluted with petroleum.
ResumenEl objetivo de este trabajo fue determinar la remoción de Cromo (VI) en solución por la biomasa celular del hongo contaminante ambiental Paecilomyces sp. por el método colorimétrico de la difenilcarbazida. La mayor bioadsorción fue a pH= 1.0±0.2, a 50 o C durante 16 horas con 7.2 mg/200 mL de concentración inicial de Cromo (VI) y 80 mg/200 mL de biomasa celular. Se concluye que la biomasa fúngica remueve adecuadamente Cromo (VI) en solución y puede utilizarse para descontaminar nichos acuáticos contaminados, ya que 1 g de biomasa fúngica remueve 100 mg/200 mL del metal a las tres horas de incubación. Palabras clave: bioadsorción de cromo, biomasa fúngica, descontaminación de aguas, colorimetría Removal of Chromium (VI) from Aqueous Solutions by Fungal Biomass of Paecilomyces sp. AbstractThe objective of this work was to determine the removal of Chromium (VI) on the Paecilomyces sp.; fungal biomass, spectrophotometrically using diphenylcarbazide as the complexing agent. The highest adsorption was obtained at pH= 1.0±0.2, at 50 o C after 16 hours of incubation, with 7.2 mg/200 mL of initial concentration of Chromium (VI) and 80 mg/200 mL of cellular biomass. It was concluded that application of this biomass on the removal of Cr (VI) in aqueous solutions can be used since 1 g of fungal biomass remove 100 mg/200 mL of this metal after three hours of incubation.
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