were plotted as the median and interquartile range (25th-75th). The Mann-Whitney nonparametric test was used to analyze numerical variables between two groups. The Kruskal-Wallis test was used to analyze numerical variables between three groups. Data analysis was performed using GraphPad Prism v 8.0 software (GraphPad Software, San Diego, CA). All comparisons were considered statistically significant at p < 0.05.
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