Adequate denture hygiene can prevent and treat infection in edentulous patients. They
are usually elderly and have difficulty for brushing their teeth.ObjectiveThis study evaluated the efficacy of complete denture biofilm removal using
chemical (alkaline peroxide-effervescent tablets), mechanical (ultrasonic) and
combined (association of the effervescent and ultrasonic) methods. Material and MethodsEighty complete denture wearers participated in the experiment for 21 days. They
were distributed into 4 groups (n=20): (1) Brushing with water (Control); (2)
Effervescent tablets (Corega Tabs); (3) Ultrasonic device (Ultrasonic Cleaner,
model 2840 D); (4) Association of effervescent tablets and ultrasonic device. All
groups brushed their dentures with a specific brush (Bitufo) and water, 3 times a
day, before applying their treatments. Denture biofilm was collected at baseline
and after 21 days. To quantify the biofilm, the internal surfaces of the maxillary
complete dentures were stained and photographed at 45º. The photographs were
processed and the areas (total internal surface stained with biofilm) quantified
(Image Tool 2.02). The percentage of the biofilm was calculated by the ratio
between the biofilm area multiplied by 100 and the total area of the internal
surface of the maxillary complete denture. ResultsThe Kruskal-Wallis test was used for comparison among groups followed by the Dunn
multiple-comparison test. All tests were performed respecting a significance level
of 0.05. Significant difference was found among the treatments (KW=21.18;
P<0.001), the mean ranks for the treatments and results for Dunn multiple
comparison test were: Control (60.9); Chemical (37.2); Mechanical (35.2) and
Combined (29.1). ConclusionThe experimental methods were equally effective regarding the ability to remove
biofilm and were superior to the control method (brushing with water). Immersion
in alkaline peroxide and ultrasonic vibration can be used as auxiliary agents for
cleaning complete dentures.
The effervescent tablets significantly reduced mutans streptococci and total aerobes from denture biofilm. However, they was not as effective against C. albicans. Ultrasonic cleansing presented a discrete antimicrobial effect and was less effective than the tablets for complete denture disinfection.
The objective of this study was to evaluate the bacterial profile in root canals of human primary teeth with necrotic pulp and periapical lesions using bacterial culture. A total of 20 primary teeth with necrotic pulp and radiographically visible radiolucent areas in the region of the bone furcation and/or the periapical region were selected. After crown access, 4 sterile absorbent paper points were introduced sequentially into the root canal for collection of material. After 30 s, the paper points were removed and placed in a test tube containing reduced transport fluid (RTF) and were sent for microbiological evaluation. Anaerobic microorganisms were found in 100% of the samples, black-pigmented bacilli in 30%, aerobic microorganisms in 60%, streptococci in 85%, Gram-negative aerobic rods in 15% and staphylococci were not quantified. Mutans streptococci were found in 6 root canals (30%), 5 canals with Streptococcus mutans and 1 canal with Streptococcus mutans and Streptococcus sobrinus. It was concluded that in root canals of human primary teeth with necrotic pulp and periapical lesions, the infection is polymicrobial with predominance of anaerobic microorganisms.
Both chlorhexidine-based treatments had a similar ability to remove denture biofilm. Immersion in 0.12% or 2.0% chlorhexidine solutions can be used as an auxiliary method for cleaning complete dentures.
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