Bacterial profile in primary teeth with necrotic pulp and periapical lesions

Abstract

The objective of this study was to evaluate the bacterial profile in root canals of human primary teeth with necrotic pulp and periapical lesions using bacterial culture. A total of 20 primary teeth with necrotic pulp and radiographically visible radiolucent areas in the region of the bone furcation and/or the periapical region were selected. After crown access, 4 sterile absorbent paper points were introduced sequentially into the root canal for collection of material. After 30 s, the paper points were removed and placed in a test tube containing reduced transport fluid (RTF) and were sent for microbiological evaluation. Anaerobic microorganisms were found in 100% of the samples, black-pigmented bacilli in 30%, aerobic microorganisms in 60%, streptococci in 85%, Gram-negative aerobic rods in 15% and staphylococci were not quantified. Mutans streptococci were found in 6 root canals (30%), 5 canals with Streptococcus mutans and 1 canal with Streptococcus mutans and Streptococcus sobrinus. It was concluded that in root canals of human primary teeth with necrotic pulp and periapical lesions, the infection is polymicrobial with predominance of anaerobic microorganisms.

Antimicrobial activity of different filling pastes for deciduous tooth treatment

“…As already described, this bacterium was used because it served as a quality parameter in sensitivity tests of antimicrobial agents, which demonstrated excellent action. 30 The resistance of the E. faecalis microorganism to antimicrobial agents, as already demonstrated, 4,28 was confirmed in this study. This finding is clinically relevant for the filling of deciduous teeth, because this organism has been found in infected root canals.…”

“…3,4 For all of these reasons, the chemical-mechanical preparation of these root canals becomes complex, requiring the reduction or elimination of any bacteria with antimicrobial irrigants and, especially, with an antimicrobial filling material of vast microbial spectrum, good diffusion and resorbability. The filling materials most commonly employed for pulp therapy in deciduous teeth are zinc oxide paste (OZE), iodoform-based paste (IBP) and calcium hydroxide (HCa), among others.…”

“…26 In spite of the proven presence of strictly anaerobic microorganisms in pathogenic pulp flora, pure aerobic and facultative anaerobic cultures were chosen, because these microorganisms are most commonly used in studies that test the antimicrobial activity of materials used in endodontic therapy. 4,27 Furthermore, studies show that the microbiota of root canals with endodontic failure is usually composed of facultative anaerobic gram-positive bacteria. 3,4,28 The bacteriostatic and bactericidal power of an antimicrobial drug should be considered in determining its potential.…”

“…4,27 Furthermore, studies show that the microbiota of root canals with endodontic failure is usually composed of facultative anaerobic gram-positive bacteria. 3,4,28 The bacteriostatic and bactericidal power of an antimicrobial drug should be considered in determining its potential. Regarding bacteriostatic power, G1 paste demonstrated this potential in all the microorganisms tested, as already shown.…”

Süt Di̇şleri̇nde Ni̇kel Ti̇tanyum Döner Aletleri̇ni̇n Kullaniminin Değerlendi̇ri̇lmesi̇

“…7 Enterococcus faecalis gibi mikroorganizmaların konvansiyonel kanal tedavisine gösterdiği direnç tedavideki başarısızlıkların önemli nedeni olmaktadır. 8 Furkasyondaki aksesuar kanallar ve atipik kök rezorbsiyonu kanalların temizlenme etkinliğini azaltmaktadır. 9 Süt dişi kanal boylarının kısa olması, aksesuar kanalların fazla sayıda bulunması, kanalların diverjan ve eğri seyretmesi, kök kanal duvarlarının ince olması, dentin dokusunun daha kolay aşındırılabil-mesi gibi kök kanal anatomisindeki farklılıklar ve fizyolojik kök rezorbsiyonu gibi durumlar süt dişi kök kanal tedavisini zorlaştırmaktadır.…”