The distribution of abnormal hemoglobins, glucose-6-phosphate dehydrogenase deficiency, blood group antigens A, B, M, N, C, c, D, E, e, V, Duffy and Diego, serum haptoglobins, transferrins and pseudocholinesterase types, were investigated in five villages of the East Coast of Mexico, where vivax malaria was endemic until recently. Hemoglobin S and G-6-PD deficiency were present i n variable degrees in all five locations. AU patients with the enzyme deficiency had the A band by electrophoresis, which coupled with the presence of hemoglobin C and haptoglobin 2-1 M in a few individuals strongly suggested Negro admixture. The high frequency of antigen V and the relatively low frequency of Fy ( a +) were congruent with the above hypothesis and the conclusion was drawn that the presence of hemoglobin S and G-6-PD deficiency in this area is due to Negro admixture. Unusually high frequencies of the Diego antigen were observed in three of the five villages studied.
Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis. In the oral cavity, clinical manifestations are considered atypical lesions. The aim of this study was to report an unusual granular ulcer secondary oral TB that does not heal, chronic, had irregular appearance with deep depression of 2 cm in diameter, and was located in buccal mucosa of the premolar area–the left mandibular arch, of a 42-year-old woman. The patient was subjected to surgical excision of ulcer in its entirety and accordingly a periodontal plastic surgery in the area of the lesion was performed positioning an autograft subepithelial connective tissue. We obtained optimal results in improving oral health, function, and patient’s comfort, in postoperative controls at 15, 30, and 60 days, respectively.
I n order to study the kinetics of erythrocyte antibody-blood group substance union, labeling of the erythrocyte antibody has frequently been used. In 1953 Boursnell, Coombs and Rizk [2] reported the results of such a study using 11s' as the identifying label. Their kinetic investigation was complicated by a nonspecific adsorption of labeled globulin molecules onto the erythrocyte surface. Using various antibody labeling procedures we have been able to fully confirm this original observation of a nonimmunologic fixation of globulin molecules t o the erythrocyte. Boursnell, Coombs and Rizk felt they were able t o eliminate this nonspecific adsorption phenomenon by the prior incubation of erythrocytes with nonlabeled globulin solution. This technique has been unsuccessful in our hands and stimulated an investigation as to the nature of the nonspecific adsorption phenomenon. The purpose of this report is to describe several quantitative characteristics of the nonimmunologic reaction of globulin molecules with the erythrocyte surface.
Methods
Human globulin containing anti-D (Rho).This material was obtained from eight individuals whose serum contained a potent, incomplete, warm-acting anti-D (Rho) antibody. The sera were precipitated with 1.4 M cold ammonium sulphate for 18 hours a t 4°C. The precipitate was washed with cold ammonium sulphate, 1.4 M, and dissolved in distilled water. The solution was dialyzed for 48 hours against 3 six liter changes of sodium chloride-borate buffer, p H 8.0.*
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