Aim: Development of a method for the isolation and purification of metagenomic RNA (mgRNA) from the ectopic bacterial flora of octopus. Methods and Results: Modifications were made to the methods of Valenzuela‐Avendaño et al. (Plant Mol Biol Rep, 2005, 23, 199a) and Chomczynski and Sacchi (Anal Biochem, 1987, 162, 156) to develop a protocol based on chemical lysis with Trizol. This proposed protocol effectively isolated mgRNA. The resulting bacterial RNA transcripts were amplified with universal primers directed to the hypervariable regions of the 16S rRNA gene by complementary DNA synthesis. Protocol efficacy in the study of metabolically active bacterial flora was proven using DGGE, which produced a banding pattern that recovered sequences mainly related to the Vibrionaceae family. Conclusion: The analysed samples were clearly complex, and the proposed protocol was proven to effectively isolate mgRNA from the metabolically active bacterial flora associated with octopus. Significance and Impact of the Study: This is the first protocol proposed for the isolation of bacterial mgRNA that allows identification and study of metabolically active bacterial flora associated with octopus. This is an important step forward in understanding and controlling the microbial community of this economically important fishery resource, aimed at detecting its potentially pathogenic bacteria.
Several investigations have demonstrated the advantageous role that plants have in the phytoremediation of soils and waters contaminated by heavy metals. Therefore, under the present investigation, Chrysopogon zizanioides and Typha latifolia plants were tested for their effectiveness in absorbing 17 heavy metals (Al,
Background: Octopus is a fishery product of economic importance worldwide, the main species caught on the coast of the Gulf of Mexico and the Caribbean Sea are Octopus maya and O. vulgaris, the first represents up to 95 % of national production. Goals: Identify the bacterial flora associated with commercial Octopus maya captured in the Yucatan Peninsula, using PCR-DGGE. Methods: From the metagenomic DNAs (mDNAs) extracted from samples representative of the octopus muscle, PCR products were synthesized with universal primers for bacteria (gc338F and 518R) and specific primers for Phylum Firmicutes (FirF: 369 and gcFirR: 1244). They were separated by electrophoresis in denaturing gradient gels (DGGE). The fragmented DNAs were recovered by elution, amplified (338F / 518R and FirF: 369 / FirR: 1244), sequenced and analyzed phylogenetically. Results: The sequences amplified with universal primers, after the DNA fragmentation by DGGE were associated with Psychrobacter urativorans, Psychrobacter sp, Pseudomonas sp, Pseudoalteromonas sp, Shewanella sp, Shewanella baltica, Klebsiella oxytoca, Vibrio aestuarianus, Photobacterium sp, Flavobacterium sp, F. antarcticum, Bizionia sp, Flavobacteriaceae bacterium, Bacillus sp, C. divergens, Cetobacterium somerae, Psychrilyobacter atlanticus, Salinimicrobium sp as well as, Flavobacteriaceae not yet classified. In the sequences amplified with specific primers (Phylum Firmicutes) were identified: Carnobacterium sp, Lactococcus piscium Lactococcus sp, and Vagococcus sp Conclusion: The bacterial genus detected have been reported in samples from marine environments; therefore, can be part of the native microbial diversity associated with commercial O. maya captured in the Yucatan Peninsula, Mexico.
Some species of pathogenic bacteria such as Salmonella, Escherichia coli, Shigella, among others, are a threat to the ichthyofauna that lives in freshwater rivers. This threat extends to the human population that develops various activities in this environment. Food products derived from these activities can affect consumers as well. Therefore, the objective of this research was to evaluate the microbiological quality of water in the middle basin of the Usumacinta River, Tabasco, Mexico. A total of 63 water samples were collected in 12 sampling sites: 36 in the dry season and 27 in the rainy season in 2013. The microbiological analysis was carried out according to Mexican standards. The highest concentration of CT was detected in Usumacinta (Usu) (130 NMP / 100mL) during the dry season and in Petunia (Pet) (240 NMP / 100mL) during the rainy season. The highest concentration of CF was detected in the water plant (Pa), Pet and Estapilla (Est) (34 NMP / 100mL), during the rainy season. The results of CT and CF registered significant differences in the Colony Forming Units (CFU), due to seasonality (p <0.05). Thus, they were not significant due to the effect of the sampling sites (p <0.05). Also, 284 putative strains of enterobacteria isolated and identified mainly Escherichia sp and Salmonella sp in the two studied seasons. These results are one of the first evidences of the microbiological quality of water in the middle basin of the river Usumacinta, Mexico.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.