The anthocyanin in `Sensation Red Bartlett' pear skin was characterized and quantified, and the effect of light quality on fruit color development was evaluated. Anthocyanin concentration was related to fruit chromaticity values. Pigments were analyzed using high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). One of two spots detected in the TLC chromatogram did not change color with molybdate sprays, indicating the possible presence of peonidin. HPLC analysis confirmed the presence of a major and a minor pigment, which co-eluted with cyanidin 3-galactoside and peonidin 3-galactoside. Monomeric anthocyanins in the pear skin extract were 6.83 mg/100 g of fruit peel. To study light quality, gelatin filters allowing passage of different wavelengths of-light were attached over the exposed side of `Sensation Red Bartlett' pears 1 month before harvest. Chromaticity was recorded before the filters were attached and after their removal at harvest using the Commission Internationale del'Eclairage (L*, a*, and b*) color space coordinates. Following color measurements, anthocyanin was extracted from individual skin disks. Skin beneath all filters yielded less hue than the control. Wavelengths that transmit above 600 nm had the largest effect on chroma, a*, and b* values. Fruit wrapped in aluminum foil to obscure all light had the highest luminosity. Wavelengths from 400 to 500 nm gave darker, less chromatic, and redder pear fruit. All treatments yielded higher anthocyanin content than the control. There was a tendency toward increased anthocyanin content with longer wavelengths. The simple linear regression of the log anthocyanin content on L* value and (a*/b*) provided an R2 = 0.41.
The upper region of the Río Negro and Neuquén valley, Argentina (latitude: 38 degrees 55 minutes South) experiences high temperatures and light intensities before the apple harvest. This hinders these fruits turning red and increases the risks of them becoming sunburnt. In the December of two growing seasons (when the fruits were about 43 mm in diameter), still some 80 days before harvest, 15% and 55% density shade nets were placed over "Fuji" apple trees. At harvest time, light distribution was determined at two canopy heights (1 and 3 m) on either side of the trees. Fruiting spurs were examined, and colour, sunburn damage, weight, soluble solid content and flesh firmness of the fruits determined. Specific leaf weight (SLW) was also established. Shade nets notably decreased the amount of photosynthetically active radiation (PAR) available; they also reduced fruit colour (redness), soluble solid content and flesh firmness, and the SLW. The 55% density net decreased fruit sunburn, but no differences were found between the 15% density net and control treatments. Spurs at the bottom of the canopy received less light, and the SLW, as well as the colour and soluble solid content of their fruit, was lower than observed for the higher spurs. The impossibility of exporting fruits damaged by high temperatures and intense solar radiation during ripening requires shade nets be used, their density depending on the conditions experienced.
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