This paper describes the effect of two weak bases (ammonium chloride and chloroquine) on the morphogenesis of Mayaro virus. When Mayaro virus-infected TC7 (monkey kidney) cells were treated with these agents it was observed that weak bases caused a signi®cant reduction in virus yield. Also, cellular protein synthesis, which is inhibited by Mayaro virus infection, recovered to nearly normal levels. However, the synthesis of Mayaro virus proteins was affected. These phenomena were dose-dependent. The process of Mayaro virus infection in vertebrate cells is very rapid. Virus precursors are not observed in cell cytoplasm and budding through the plasma membrane seems to be the only way of virus release. Electron microscopy of cells infected with Mayaro virus and treated with weak bases revealed an accumulation of virus structures in cell cytoplasm. The study also noted an inhibition of budding through the plasma membrane and the appearance of virus particles inside intracytoplasmic vacuoles. These observations indicate an impairment at the ®nal stages of the virus replication cycle.
Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.
y unitermos key words resumoPaciente masculino de 29 anos, portador de um quadro febril acompanhado de exantema generalizado, com suspeita de roseola infantum, apresentou desconforto e hiperemia do olho direito, além de lesões labiais vesiculares. Através da técnica da PCR diagnosticou-se que a lesão labial fora provocada pelo vírus do herpes simples e a lesão ocular produzida pelo vírus da varicela-zoster. A reação em cadeia da polimerase (PCR) é uma técnica de altas sensibilidade e especificidade, que permite a detecção de quantidades mínimas de DNA ou RNA. A PCR vem sendo utilizada como auxiliar do diagnóstico clínico, terapia, classificação e estudos epidemiológicos de doenças. Esta técnica permite um resultado geralmente mais rápido que os métodos tra- VZV HSV Roseola infantum IntroduçãoVários membros da família Herpesviridae são pató-genos importantes para o homem. Após a infecção primária, muitas vezes assintomática, ocorre o estabelecimento da latência, geralmente nos neurônios sensitivos. Entre os vírus desta família salientaremos o ví-rus do herpes simples dos tipos 1 e 2 (HSV 1/2), o vírus da varicela-zoster (VZV) e o herpesvírus humano 6 (HHV-6).O herpesvírus humano 6, cuja infecção primária geralmente ocorre na infância, é o agente etiológico da roseola infantum. Como todo vírus neurotrópico, pode levar a convulsões febris e encefalites (1). O vírus do herpes simples (HSV), como é sabido, provoca uma série de manifestações clínicas, estando a orofaríngea entre as mais comuns. Na infecção primária observamos febre, faringite, lesões vesiculares e ulcerativas, entre outros sinais e sintomas. Quando ocorre a reci- RELATO DE CASO CASE REPORT
We have previously observed that interferon (recIFNa2b) blocks the process of morphogenesis of Mayaro virus in TC7 cells (monkey kidney). In this work we show that IFNa inhibits preferentially virus glycoproteins and their precursors, and this effect is probably correlated to the alterations in the morphogenesis process previously observed.
Observamos anteriormente que o Interferon (IFN) recombinante a2b bloqueia o processo de morfogênese do vírus Mayaro em células TC7 (rim de macaco). Neste trabalho demonstramos que o IFNa inibe preferencialmente as glicoproteínas virais e seus precursores e que este efeito está, provavelmente, correlacionado com as alterações no processo de morfogênese previamente observadas
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