BeAn 58058 virus (BAV) was isolated from an Oryzomis rodent in Brazil. BAV was shown to be antigenically related to another poxvirus also isolated in Brazil, the Cotia virus, but it remained ungrouped. Electron microscopy revealed that BAV has a typical poxvirus morphology. The Hind III DNA profile of BAV genome was similar with that of VV WR and Lister, but some differences in the profile were detected. We have also detected the presence of genes homologous to vaccinia virus (VV WR) genes in the genome of BAV. Genes related to vaccinia thymidine kinase (TK) gene and vaccinia growth factor (VGF) gene were found. The patterns of TK and VGF mRNA transcripts described for vaccinia virus infected cells were observed in BAV infected cells. Nucleotide sequence of BAV VGF homologous gene was similar to VV WR VGF sequences. This similarity was further seen when cross-hybridization of total genomes of BAV and VV was done. Polypeptide synthesis of BAV and vaccinia in infected cells also showed similar profiles. The genetic data was used to construct a phylogenetic tree where BAV and VV were placed at the same cluster. Based on our findings we propose that BAV is a vaccinia virus variant.
Oito isolados de fungos micorrízicos rizoctonióides foram obtidos do sistema radicular de orquídeas neotropicais, a saber: Bulbophyllum weddelii (Lindl.) Rchb. f., Epidendrum dendrobioides Thunb., Maxillaria acicularis Herb. ex Lindl., Oncidium gracile Lindl., Pleurothallis teres Lindl., Prosthechea vespa (Vell.) W.E. Higgins, Sophronitis milleri (Blumensch. ex Pabst) C. Berg & M.W. Chase e Sarcoglottis sp., que ocorrem em campos rupestres da região do Quadrilátero Ferrífero, no Estado de Minas Gerais, Brasil. Três gêneros anamórficos foram identificados: Epulorhiza, isolados do sistema radicular de E. dendrobioides e S. milleri; Ceratorhiza, isolados de B. weddelii, O. gracile, P. teres e P. vespa e Rhizoctonia, isolados de M. acicularis e Sarcoglottis sp. O trabalho constitui-se no primeiro relato taxonômico e de caracterização morfológica de fungos micorrízicos rizoctonióides associados a espécies de orquídeas que ocorrem em campos rupestres no Brasil.
In the present study, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was monitored in environmental samples from rural and vulnerable areas (a presidio, worker accommodation units, and river waters upstream and downstream of a rural community) from Minas Gerais State region, Southern Brazil, in August 2020. The sampling was performed prior to official declaration of the coronavirus disease (COVID-19) cases in those sites. SARS-CoV-2 RNA was detected in the presidio and workers accommodation units (3.0 × 10 4 virus genome copies (GC)/mL and 4.3 × 10 4 GC/mL of sewage, respectively). While SARS-CoV-2 was not detected in the river water upstream of the rural community, SARS-CoV-2 RNA was detected in downstream river waters (1.1 × 10 2 SARS-CoV-2 GC/mL). The results obtained in this study highlight the utility of SARS-CoV-2 monitoring in wastewater and human sewage as a non-invasive early warning tool to support health surveillance in vulnerable and remote areas, particularly in development countries.
RESUMO Esta pesquisa científica teve como principal objetivo identificar patótipos deEscherichia coli diarreiogênica nas águas superficiais da Bacia Hidrográfica do Rio Xopotó, na região do Alto Rio Doce, Minas Gerais. Os estudos referentes às estirpes diarreiogênicas de E. coli no meio ambiente no Brasil são escassos.A bacia hidrográfica escolhida para o estudo sofre intensa degradação ambiental devido ao lançamento de esgoto in natura em seus corpos d' água e às atividades antrópicas, como a agropecuária. As coletas de água nos
The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct operational phases, when the reactor were stable (low variation of color removal), to assess the effect of glucose and yeast extract as source of carbon and redox mediators, respectively. Reactors performance was evaluated based on COD (chemical oxygen demand) and color removal. The microbial dynamics were investigated by PCR-DGGE (Polimerase Chain Reaction - Denaturing Gradient of Gel Electrophoresis) technique by comparing the 16S rDNA profiles among samples. The results suggest that the composition of microorganisms changed from the beginning to the end of the reactor operation, probably in response to the presence of azo dye and/or its degradation byproducts. Despite the highest efficiency of color removal was observed in the presence of 500 mg/L of yeast extract (up to 93%), there were no differences regarding the microbial profiles that could indicate a microbial selection by the yeast extract addition. On the other hand Methosarcina barkeri was detected only in the end of operation when the best efficiencies on color removal occurred. Nevertheless the biomass selection observed in the last stages of UASB operation is probably a result of the washout of the sludge in response of accumulation of aromatic amines which led to tolerant and very active biomass that contributed to high efficiencies on color removal.
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