The objective of this study was to adapt and investigate validity evidence for the Team Psychological Safety Survey for Brazil and to test its feasibility to emerge at the team level. A sample of 8,310 female workers answered the scale. Initial analyses indicated the single-factor solution fitness, with Cronbach’s alpha = 0.84. Confirmatory factor analysis model obtained good fitness coefficients, CFI = 0.995, RMSEA = 0.07. Emersion was viable due to group variance identified by and ICC calculations. The hypothesis stated that psychological safety correlates with perceived organizational practices and that differences exist between men and women’s practices. Findings support that the good practices positively related to psychological safety, with differences between gender, while a negative relationship with bad practices was partially confirmed. A quadratic trend was identified between organizational status and psychological safety. Results provide validity evidence for the survey for samples of Brazilian women.