The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to
R
gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete
Phytophthora infestans.
Quantitative disease resistance (QDR), as any other quantitative trait in plants, can be genetically mapped to understand the genetic architecture. Association mapping using DNA-based markers has been implemented in many crops to dissect quantitative traits. We used an association mapping approach with candidate genes to identify the first genes associated with quantitative resistance to late blight in
Solanum tuberosum
Group Phureja. Twenty-nine candidate genes were selected from a set of genes that were differentially expressed during the resistance response to late blight in tetraploid European potato cultivars. The 29 genes were amplified and sequenced in 104 accessions of
S. tuberosum
Group Phureja from Latin America. We identified 238 SNPs in the selected genes and tested them for association with resistance to late blight. The phenotypic data were obtained under field conditions by determining the area under disease progress curve (AUDPC) in two seasons and in two locations. Two genes were associated with QDR to late blight, a potato homolog of thylakoid lumen 15 kDa protein (
StTL15A
) and a stem 28 kDa glycoprotein (
StGP28
).
Key message
: A first association mapping experiment was conducted in
Solanum tuberosum
Group Phureja germplasm, which identified among 29 candidates two genes associated with quantitative resistance to late blight.
In this paper the problem of creating a wireless infrastructure to control a vertical transportation system is addressed. The work assumes the use of the standard IEEE 802.15.4 as wireless control field bus. Throughout the paper different network topologies are analyzed together with the network management algorithms. As a result of this analysis the tree topology is selected and experimentally verified as the most suitable for the vertical transportation system.
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