Community on-site separation of wastewater is a treatment approach that leads to more efficient processes, with former pollutants considered resources to be recycled.Black water has high organic matter content and can be a suitable feedstock for anaerobic treatment systems. Biological methane production (BMP) tests were conducted using Plackett-Burman design to screen the effects of adding Fe, Ni, Cu, Co, Mn, Ba and Se, with simulated black water (SBW) as the substrate. In the inoculum, most metals were found mainly in the organic matter/sulfide and residual fractions except for Mn, which was present at 12.3% in the bioavailable fractions (exchangeable and carbonates), and Ba, which was evenly distributed among all the fractions. Under the studied ranges of added metals, Fe, Ni, and Co did not show a clear effect on the anaerobic digestion of the black water. Ba had a significant negative effect on methane production and Mn addition enhanced the toxic effect. A specific methanogenic activity (SMA) between 18 and 27% lower than the control, 11.17 mLCH4.gCOD-1.d , respectively.
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