This study was conducted to evaluate the microbiological status of cheese made from unpasteurized cows’ milk, to examine the safety of the cheese and to observe the changes that occurred in its microbial community during ripening and storage. Furthermore, the pH, the moisture and salt concentration were also monitored throughout processing, ripening and storage. Seven cheesemaking trials took place along with the microbiological and physicochemical analysis of the milk, curd and cheese produced. The milk used for the cheesemaking, two curd samples before the heating and two after the heating, two cheese samples at days 3, 7, 15, 30, 60 and 90 were subjected to microbiological analysis for total mesophilic bacterial count (for milk only), Enterobacteriaceae, E. coli, Staphylococcus, Salmonella, Listeria, and Clostridium. The microbiological quality of raw milk was found to be good. It was initially slightly above the EU limit but improvements associated with farm biosecurity and milking equipment hygiene led to a significantly improved milk quality. A small increase in the prevalence of indicator microorganisms in curd and cheese samples was observed for the first few days, followed by a relatively stable condition as manufacturing proceeded and throughout the ripening of the final product. In two cheesemaking trials, Clostridium perfringens and Salmonella spp. were detected, the first originating from the milk and the second from the environment. The use of good-quality raw milk under sanitary conditions, the application of good manufacturing practices and a maturation period in a controlled environment were found to be the necessary prerequisites for the production of safe raw cheese products.

Background The impacts of climate change on crop and livestock sectors are well-documented. Climate change and its related events (e.g., high temperatures, extreme events, disease outbreaks) affect livestock production in various ways (e.g., nutrition, housing, health, welfare), and tend to compromise the physical productivity and the economic performances. Understanding animal responses to climate change may help planning strategies to cope with the adverse climatic conditions and also to reduce polluting emissions. Through an interdisciplinary approach, we develop a conceptual framework to assess and develop new organisational models for Mediterranean small-scale farming systems so as to mitigate the impacts of climate change, to improve farm management and farming technologies, and to achieve an effective adaptation to the climate changes. The conceptual framework consists of four phases: (i) community engagement, (ii) strategies development, (iii) data collection and analysis, (iv) business model generation and sustainability assessment. We assess strengths, weaknesses, opportunities, and threats of the eco-solutions by mean of a SWOT (Strengths, Weaknesses, Opportunities, Threats) analysis technique. Results The proposed eco-solutions are expected to increase the sustainability of agriculture and food production systems by introducing new and efficient uses of natural resources. The proposed models are expected to have an impact not only on the environment (in terms of mitigation), but also on the economic and social performances, as they are expected to foster the responses of small-scale farms to the increasingly frequent effects of climate change (adaptation solutions). Among the positive impacts, we emphasise the importance of more stable revenues, a tendency that would help farmers to raise their revenues. Last but not least, we found that the proposed models are likely to increase the social resilience of the farming systems to the challenges imposed by the climate change. Conclusions The eco-solutions can support stakeholders involved in Mediterranean small-scale farming systems by suggesting novel land, crop, and livestock management approaches to optimise revenue flows, business models and climate change mitigation strategies thanks to the adoption of a systemic approach, that is not only focused on specific components of the system but instead based on the linkages between environmental, social, and economic aspects.

The aim of the present study was to characterize LAB isolates from raw-milk cheeses, to evaluate some of their technological properties and to select a few ‘wild’ LAB strains that could potentially be used as starter cultures. LAB strains were isolated and identified from raw milk, curd, and cheese at 30, 60, and 90 days of ripening. A total of 100 strains were isolated, 20 from each phase of ripening. All isolates were tested for acidification ability, curd formation, and aroma production at 32 °C and 42 °C after 24 and 48 h. Following the acidification test, 42 strains were selected for identification and characterization of their technological properties. A high proportion of lactic acid bacteria and Gram + cocci were found throughout the cheese-making process. Enterococci reached their maximum proportion on the 7th day of ripening while Lactobacilli increased significantly during the first month of ripening. Forty-two strains were identified by phenotypic, biochemical, and molecular techniques. Lactococci were predominant in raw milk and curd while Lactobacilli in the ripening of the cheese. Four LAB strains including one Leuconostoc pseudomenteroides, two Lacticaseibacillus paracasei subsp. paracasei and one Enterococcus hirae, were proposed for their potential use as starters or secondary cultures.

An increased consumer interest in goat milk has been registered in recent years due to its health-related benefits. This study aimed to investigate the effects of production systems and seasons on fatty-acid composition and nutritional indices for the healthy fat consumption of retail goat milk. The study lasted one year, and milk samples (n = 160) from 14 brands (10 conventional and 4 organic) were collected on a monthly basis. Organic milk had a marginally lower fat and protein content compared with conventionally produced milk according to the declared chemical composition. The production system did not significantly affect milk fatty-acid composition and its nutritional value, indicating that there were no major differences in the feeding strategies between conventionally and organically raised ewes. Seasonal effects were observed in the fatty-acid profile and the nutritional indices. Lower content of saturated fatty acids (SFAs), hypocholesterolemic/hypercholesterolemic ratio (h/H) and linoleic acid/α-linolenic acid (LA/ALA) ratio values were observed in summer. Retail goat milk had a desirable nutritional value, irrespective of production system and season, due to the following characteristics: low atherogenic index (AI) and LA/ALA ratio values, and high h/H ratio, desirable fatty acid (DFA) values and health-promoting index values. In conclusion, neither production nor season significantly impacted the fatty-acid composition and the fat-consumption nutritional indices of retail goat milk produced in Greece.