No abstract

The New Zealand stick insect Clitarchus hookeri has both sexual and parthenogenetic (all-female) populations. Sexual populations exhibit a scramble competition mating system with distinctive sex roles, where females are signalers and males are searchers, which may lead to differences in the chemical and morphological traits between sexes. Evidence from a range of insect species has shown a decay of sexual traits is common in parthenogenetic lineages, especially those traits related to mate attraction and location, presumably due to their high cost. However, in some cases, sexual traits remain functional, either due to the recent evolution of the parthenogenetic lineage, low cost of maintenance, or because there might be an advantage in maintaining them. We measured morphological and chemical traits of C. hookeri to identify differences between males and females and between females from sexual and parthenogenetic populations. We also tested the ability of males to discriminate between sexual and parthenogenetic females in a laboratory bioassay. Our results show that male C. hookeri has morphological traits that facilitate mobility (smaller body with disproportionately longer legs) and mate detection (disproportionately longer antennae), and adult females release significantly higher amounts of volatile organic compounds than males when this species is sexually active, in accordance with their distinctive sex roles. Although some differences were detected between sexual and parthenogenetic females, the latter appear to maintain copulatory behaviors and chemical signaling. Males were unable to distinguish between sexual and parthenogenetic females, suggesting that there has been little decay in the sexual traits in the parthenogenetic lineage of C. hookeri.

Brachaspis nivalis, Sigaus australis and Paprides nitidus are grasshopper species endemic to Aotearoa, New Zealand where they are sympatric in several regions of South Island. On mountains of Kā Tiritiri o te Moana (Southern Alps), B. nivalis is more abundant on scree/rock habitat, whereas S. australis and P. nitidus are prevalent in alpine tussock and herbfields. It is expected, therefore, that these species have different sensory needs that are likely to be apparent in the type, abundance, and distribution of chemo-sensilla on their antennae. It is also likely that natural selection has resulted in sexual differences in sensilla. To test these hypotheses, abundance and distribution of the chemo-sensilla on the dorsal and ventral surfaces of their antennae were characterized in adult males and females of the three species. Five types of chemo-sensilla were identified on the distal portion of their antenna: chaetica, basiconica, trichoidea, coeloconica, and cavity. All species had significantly more chemo-sensilla on the ventral than the dorsal surface of antennae and a similar distribution pattern of chemo-sensilla. Despite having relatively short antenna, B. nivalis had the largest number of olfactory sensilla, but the fewest chaetica of the three species studied. A plausible explanation is that B. nivalis is abundant on  less vegetated habitats compared to the other species, and therefore may rely more on olfaction (distance) than gustatory (contact) reception for finding food. No significant differences were observed between the sexes of B. nivalis and P. nitidus, however, S. australis males had significantly more basiconica sensilla than females.

Brachaspis nivalis, Sigaus australis and Paprides nitidus are grasshopper species endemic to Aotearoa New Zealand where they are sympatric in several regions of South Island. On mountains of Kā Tiritiri o te Moana (Southern Alps), B. nivalis is most abundant on scree/rock habitat whereas S. australis and P. nitidus are prevalent in alpine tussock and herbfields. It is expected, therefore, that these species have different sensory needs that are likely to be apparent in the type, abundance, and distribution of chemo-sensilla on their antennae. It is also likely that sexual selection has resulted in sex linked differences in sensilla. To test these hypotheses, abundance and distribution of the chemo-sensilla on the dorsal and ventral surfaces of their antennae were characterized in adult males and females of the three species. Five types of chemo-sensilla were identified on the distal portion of their antenna: chaetica, basiconica, trichoidea, coeloconica, and cavity. All species had significantly more chemo-sensilla on the ventral than the dorsal surface of antennae and a similar distribution pattern of chemo-sensilla. Despite having relatively short antenna, B. nivalis had the largest number of olfactory sensilla, but the fewest chaetica of the three species studied. A plausible explanation is that B. nivalis prefer less vegetated habitats compared to the other species, and therefore may rely more on olfaction (distance) than taste (contact) reception for finding food. No significant differences were observed between the sexes of B. nivalis and P. nitidus, however, S. australis males had significantly more basiconica sensilla than females.