Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
The field of mammalian synthetic
biology is expanding quickly,
and technologies for engineering large synthetic gene circuits are
increasingly accessible. However, for mammalian cell engineering,
traditional tissue culture methods are slow and cumbersome, and are
not suited for high-throughput characterization measurements. Here
we have utilized mammalian cell-free protein synthesis (CFPS) assays
using HeLa cell extracts and liquid handling automation as an alternative
to tissue culture and flow cytometry-based measurements. Our CFPS
assays take a few hours, and we have established optimized protocols
for small-volume reactions using automated acoustic liquid handling
technology. As a proof-of-concept, we characterized diverse types
of genetic regulation in CFPS, including T7 constitutive promoter
variants, internal ribosomal entry sites (IRES) constitutive translation-initiation
sequence variants, CRISPR/dCas9-mediated transcription repression,
and L7Ae-mediated translation repression. Our data shows simple regulatory
elements for use in mammalian cells can be quickly prototyped in a
CFPS model system.
Biopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is currently more expensive than the production of oil-derived plastics, and therefore, more efficient P(3HB) production processes would be desirable. In this study, we describe the model-guided design and experimental validation of several engineered P(3HB) producing operons. In particular, we describe the characterization of a hybrid phaCAB operon that consists of a dual promoter (native and J23104) and RBS (native and B0034) design. P(3HB) production at 24 h was around six-fold higher in hybrid phaCAB engineered Escherichia coli in comparison to E. coli engineered with the native phaCAB operon from Ralstonia eutropha H16. Additionally, we describe the utilization of non-recyclable waste as a low-cost carbon source for the production of P(3HB).
In contrast to the versatility of regulatory mechanisms in natural systems, synthetic genetic circuits have been so far predominantly composed of transcriptionally regulated modules. This is about to change as the repertoire of foundational tools for post-transcriptional regulation is quickly expanding. We provide an overview of the different types of translational regulators: protein, small molecule and ribonucleic acid (RNA) responsive and we describe the new emerging circuit designs utilizing these tools. There are several advantages of achieving multilevel regulation via translational switches and it is likely that such designs will have the greatest and earliest impact in mammalian synthetic biology for regenerative medicine and gene therapy applications.
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