Nagy, E., Petterson, M. & MArdh, P-A. Antibiosis between bacteria isolated from the vagina of women with and without signs of bacterial vaginosis. APMIS 99: 739-744, 1991. Lactobacilli from women with and without bacterial vaginosis (BV) were tested for HzOz production. Thirty-seven (79%) of the 47 strains of lactobacilli isolated from the women without BV produced HzOz, while only nine (23%) of the 39 strains of lactobacilli obtained from women with BV did so. Five of 20 H,O,-producing and two of 26 non-producing strains of Lactobacillus exhibited antibiosis against four of 12 strains of peptostreptococci and two of 10 strains of Mobiluncus spp. None of a further 41 different anaerobic and facultative anaerobic bacterial strains were inhibited by any of the isolates of lactobacilli tested. Some strains of Gardnerella vaginalis, Bacteriodes spp., Mobiluncus spp. and Peptostreptococcus spp. inhibited the growth of three strains of lactobacilli belonging to different species. When the pH of the culture medium was increased from 6.0 to 6.5 this led to a decrease in the number of strains inhibited and/or the size of the growth-inhibitory zones. Different concentrations of H202 did not inhibit any of the strains tested. The growth-inhibitory effect of lactobacilli could not be related to their bacteriocin production. Increasing the iron content of the medium by adding FeCl, (0.01 mM-1 mM) decreased or completely abolished the antibiosis.
A study of the effect of human breast milk, and components thereof, on the capacity of Chlamydia trachomatis to form inclusions in cycloheximide‐treated McCoy cells, was undertaken. Pooled whole milk collected during the first week of breast feeding caused a concentration‐dependent inhibition of the chlamydial inclusion‐formation. The activity resided in the fat and fat globule membrane (FGM) components of the milk. The active principle in the FGM fraction is heat‐stable and pronase‐sensitive, but resistant to both neuraminidase and periodate. Immunoglobulins was not responsible for the inhibition. Whey and casein fractions of milk increased the chlamydial inclusion‐formation. The activity of the whey was heat‐stable, dose‐related, and had a mol.wt. of 12,000. The casein fraction was still active after heat treatment. Whey samples collected up to 28 days after delivery varied slightly in their stimulatory activity, with an optimum between the 7th and 14th days. The present study demonstrated a multieffect of breast milk on chlamydial inclusion‐formation: an inhibitory activity due to a protein compound as well as another factor in the fat fraction and an enhancing effect due to a heat‐stable factor(s).
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