The present study aimed to determine the diagnostic value of the serum levels and mutational status of IL-8, IL-27 and VEGF, and the expression levels of human ether-a-go-go-related gene (hERG) in patients with colorectal cancer (CRC). The serum concentrations were determined using the ELISA technique and genotype variations of IL-8, IL-27 and VEGF were examined using Sanger sequencing, and the expression levels of hERG, which encodes a potassium channel, were determined by quantitative PCR, in blood and tissue samples obtained from 80 patients with CRC and 80 healthy individuals. The results of the present study revealed that the percentage of granulocytes and serum concentrations of carcinoembryonic antigen, IL-8 and IL-27 were significantly increased, whereas the percentage of lymphocytes was decreased in patients with CRC. In total, 31 mutations in three genes (eight mutations in VEGF, 13 mutations in IL-27 and 10 mutations in IL-8) were identified in patients with CRC. The relative mRNA expression levels of hERG were also significantly upregulated in tissue and blood samples of patients with CRC compared with those of healthy individuals. In conclusion, the results of the present study indicated that the increased concentrations and genetic variations of IL-8, IL-27 and VEGF may serve important roles in the development and angiogenic processes of CRC. These changes were concomitant with the upregulation of the expression levels of the potassium channel hERG.
Colon cancer is the third most common cancer in the world, with an estimated incidence of 72,090 cases among men and 70,480 among women in the United States each year. It is also the third leading cause of cancer death with approximately 26,580 deaths of men and 24,790 deaths of women each year [1]. The gross incidence of colorectal cancer among Algerian men doubled between 2008 and 2015. In 2015, the incidence of colorectal cancer in men is getting closer and closer to that of the lung, and prostate cancer is returning to the third place before bladder cancer. Colorectal cancer is the second most common cancer in Algiers after breast cancer [2]. There are proofs that the right colon cancer (RCC) is different from left colon cancer (LCC) and rectal cancer. There are differences in embryological origins, as well as anatomical, histological, genetic and immunological differences between RCC and LCC [1,3]. The segments of the proximal and distal colon have different embryological origins. The first develops from the midgut and the last from the posterior intestine. Both are physiologically different [4]. The main treatment for colon cancer is surgical resection. The different types of resection depend on the location of the tumor are right hemicolectomy, enlarged right hemicolectomy, left hemicolectomy and left and right segmental colectomies. The same oncologic findings were obtained in the literature for both classical and laparoscopic approaches. RCCs are associated with iron deficiency anemia, late stage and advanced age. In addition, RCC has a tendency to involve large polypoid lesions, exophytic, developing in the lumen of the colon. In contrast, LCCs tend to include invasive constrictive lesions encircling the lumen and causing obstruction. [5] Tumor samples also showed
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.