Mosquitoes are vectors of various pathogens that cause diseases in humans and animals. To prevent the outbreak of mosquito-borne diseases, it is essential to control vector populations, as treatment or vaccination for mosquito-borne diseases are often unavailable. Insect-specific viruses (ISVs) have previously been described as being potentially helpful against arboviral disease outbreaks. In this study, we present the first in vivo characterization of the ISV Culex Y virus (CYV). CYV was first isolated from free-living Culex pipiens mosquitoes in 2010; then, it was found in several mosquito cell lines in a further study in 2018. For mammalian cells, we were able to confirm that CYV does not replicate as it was previously described. Additionally, we found that CYV does not replicate in honey bees or locusts. However, we detected replication in the Culex pipiens biotype molestus, Aedes albopictus, and Drosophila melanogaster, thus indicating dipteran specificity. We detected significantly higher mortality in Culex pipiens biotype molestus males and Drosophila melanogaster, but not in Aedes albopictus and female Culex pipiens biotype molestus. CYV could not be transmitted transovarially to offspring, but we detected venereal transmission as well as CYV in mosquitos’ saliva, indicating that an oral route of infection would also be possible. CYV’s dipteran specificity, transmission routes, and killing effect with respect to Culex males may be used as powerful tools with which to destabilize arbovirus vector populations in the future.

The current rapidly advancing climate change will affect the transmission of arthropod-borne viruses (arboviruses), mainly through changes in vector populations. Mosquitos of the Culex pipiens complex play a particularly prominent role in virus transmission in central Europe. Factors that contribute to the vector population density and the ability of those vectors to transmit viral pathogens (vector competence) can include nutrition during the larval stages. To test the influence of larval diet on larval survival and adult emergence, as well as vector competence, several diets varying in their nutritional composition were compared using a newly established assay. We tested the effects of 17 diets or diet combinations on the fitness of third-instar larvae of Culex pipiens biotype molestus. Larval survival rates at day 7 ranged from 43.33% to 94.44%. We then selected 3 of the 17 diets (Tetra Pleco, as the routine feed; JBL NovoTab, as the significantly inferior feed; and KG, as the significantly superior feed) and tested the effect of these diets, in combination with Culex Y virus infection, on larval survival rate. All Culex Y virus-infected larvae showed significantly lower larval survival, as well as low pupation and adult emergence rates. However, none of the tested diets in our study had a significant impact on larval survival in combination with viral infection. Furthermore, we were able to correlate several water quality parameters, such as phosphate, nitrate, and ammonium concentration, electrical conductivity, and low O2 saturations, with reduced larval survival. Thus, we were able to demonstrate that Culex Y virus could be a suitable agent to reduce mosquito population density by reducing larval density, pupation rate, and adult emergence rate. When combined with certain water quality parameters, these effects can be further enhanced, leading to a reduced mosquito population density, and reduce the cycle of transmission. Furthermore, we demonstrate, for the first time, the infection of larvae of the mosquito Culex pipiens biotype molestus with a viral pathogen.