The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may contribute to improve virus diagnosis by accurately quantifying virus titre variations in grapevines.
RESUMO Vírus sorologicamente relacionado com o Tomato ringspot virus encontrado em amoreiras-pretas no Rio Grande do SulVírus relacionado sorologicamente ao Tomato ringspot virus (ToRSV) foi detectado em plantas de juá (Solanum viarum) procedente de Vacaria (Campos de Cima da Serra), RS, mostrando forte mosaico e presentes nas entrelinhas de amoreiras-pretas. Foi transmitido mecanicamente desta invasora e de amoreiras-pretas (Rubus sp.) para Chenopodium quinoa, na qual induziu distorções foliares e distorção apical do caule, enfezamento geral, manchas diminutas cloróticas e necróticas nas folhas. O vírus em extratos foliares reagiu positivamente em DAS-ELISA com um anti-soro comercial contra o isolado Peach yellow bud mosaic (PYBM) de ToRSV mas não com um anti-soro do isolado Chickadee (CH) do mesmo vírus. As amoreiras-pretas infetadas não mostravam sintomas perceptíveis.
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