ABSTRACT1. The complex life cycle of endangered European freshwater pearl mussel Margaritifera margaritifera L. involves an obligatory parasitic phase on a host fish. Knowledge on the host-parasite interaction and on the suitability of different host fish species and strains is required both for the management of wild fish and mussel populations as well as for improving the efficiency of captive breeding methods.2. In this study, the suitability of different salmonid strains for hosting glochidia was tested, including Danube salmon (Hucho hucho L.) and three brown trout (Salmo trutta L.) strains from inside and outside the freshwater pearl mussel distribution range. All brown trout strains as well as Danube salmon were successfully infected with freshwater pearl mussel glochidia and encystment of mussel larvae was detected.3. One brown trout strain originating from the natural pearl mussel distribution range was identified as the most suitable host, revealing the highest fish-weight-normalized infection rates and highest glochidial growth rates, whereas endemic Danube salmon was least suitable. Under natural conditions, the role of Danube salmon may be attributed to the long-distance dispersal of glochidia in the Danube system, whereas sedentary brown trout appear to be the most important hosts at a local scale.4. Successful infection of suitable hosts and the maintenance of these host-parasite systems in calcareous water were demonstrated in this study. These results indicate that neither the infection process nor the encystment phase of freshwater pearl mussels is dependent on low lime concentrations.5. The results of this study suggest that careful selection and management of appropriate host fish strains is mandatory for sustainable conservation management of freshwater pearl mussel populations.

The introduction of fine sediments into streams is considered to have a major effect on habitat quality affecting the reproduction of sensitive species such as unionid mussels and salmonid fishes. To date, there is a lack of information on the magnitude and spatio-temporal resolution of sediment introduction.This study aimed to quantify the spatio-temporal deposition of fine sediments in headwater streams in relation to the status of Margaritifera margaritifera and Salmo trutta. Fine sediment deposition was linked to physicochemical conditions of the adjacent streambed.The mean observed deposition of fine sediments over the study period was 3.4 kg m À2 month À1 with a high spatio-temporal variation ranging from <0.01 to 20.3 kg m À2 month À1 . Discharge had the strongest influence on deposition rates.Mean differences in redox potential between free-flowing water and the interstitial zone were 90 mV. The spatio-temporal variability of physicochemical parameters increased with degree of degradation. High-quality reaches had more constant conditions.Our results indicate that monitoring of sediment quality and deposition in streams has to comprise several time points and study reaches, or should at least be conducted during periods with the most adverse habitat conditions, to allow valid assessments of habitat quality. In streams with increased fine sediment deposition, in-stream restoration measures are insufficient for the enhancement of pearl mussel habitats as a result of rapid clogging of interstitial pores. Only integrative catchment management based on detailed habitat analysis can ensure sufficient habitat quality for species sensitive to siltation.

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Freshwater mussels of the order Unionoida have life cycles that include larval attachment to and later metamorphosis on suitable host fishes. Information on the trophic relationship between unionoid larvae and their host fishes is scarce. We investigated the trophic interaction between fish hosts and encysted larvae of two species of freshwater mussels, Margaritifera margaritifera and Unio crassus, using stable isotope analyses of larvae and juvenile mussels as well as of host fish gill and muscle tissues before and after infestation. Due to different life histories and durations of host-encystment, mass and size increase in M. margaritifera during the host-dependent phase were greater than those of U. crassus. d 13 C and d 15 N signatures of juvenile mussels approached isotopic signatures of fish tissues, indicating a parasitic relationship between mussels and their hosts. Shifts were more pronounced for M. margaritifera, which had a five-fold longer host-dependent phase than U. crassus. The results of this study suggest that stable isotope analyses are a valuable tool for characterizing trophic relationships and life history strategies in host-parasite systems. In the case of unionoid mussels, stable isotopic shifts of the larvae are indicative of the nutritional versus phoretic importance of the host.Additional key words: parasitism, trophic transfer, Margaritifera margaritifera, Unio crassus Invertebrate Biology 134(2): 129-135.

Substratum quality and oxygen supply to the interstitial zone are crucial for the reproductive success of salmonid fishes. At present, degradation of spawning grounds due to fine sediment deposition and colmation are recognized as main factors for reproductive failure. In addition, changes in water temperatures due to climate change, damming, and cooling water inlets are predicted to reduce hatching success. We tested the hypothesis that the biological effects of habitat degradation depend strongly on the species-specific spawning seasons and life-history strategies (e.g., fall- vs. spring-spawners, migratory vs. resident species) and assessed temperature as an important species-specific factor for hatching success within river substratum. We studied the species-specific differences in their responses to such disturbances using egg-to-fry survival of Danube Salmon (Hucho hucho), resident brown trout (Salmo trutta fario), and migratory brown trout (Salmo trutta lacustris) as biological endpoint. The egg incubation and hatching success of the salmonids and their dependence on temperature and stream substratum quality were compared. Hatching rates of Danube salmon were lower than of brown trout, probably due to higher oxygen demands and increased interstitial respiration in spring. Increases in maximum water temperature reduced hatching rates of resident and migratory brown trout (both fall-spawners) but were positively correlated with hatching rates of Danube salmon (a spring-spawner). Significantly longer incubation periods of resident and migratory brown trout coincided with relatively low stream substratum quality at the end of the egg incubation. Danube salmon seem to avoid low oxygen concentrations in the hyporheic zone by faster egg development favored by higher water temperatures. Consequently, the prediction of effects of temperature changes and altered stream substratum properties on gravel-spawning fishes and biological communities should consider the observed species-specific variances in life-history strategies to increase conservation success.

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Please cite this article as: Denic, M., Tauebert, J.-E., Lange, M., Thielen, F., Scheder, C., Gumpinger, C., Geist, J.,Influence of stock origin and environmental conditions on the survival and growth of juvenile freshwater pearl mussels (Margaritifera margaritifera) in a cross-exposure experiment, Limnologica (2014), http://dx. AbstractThe freshwater pearl mussel (Margaritifera margaritifera) is a highly specialized and sensitive freshwater bivalve, whose survival in the juvenile phase is indicative of high quality habitats. This contribution investigates the use of juvenile freshwater pearl mussels as bioindicators, considering the influence of mussel stock and study stream conditions on juvenile performance, as described by survival and growth rates. A standardized cross experiment was carried out investigating juvenile performance in four different pearl mussel stocks originating from the Rhine, Danube and Elbe drainages, representing distinct genetic conservation units. The juveniles were exposed in five study streams which were selected to integrate pearl mussel streams with different water qualities and recruitment status of the mussel population. Per study stream, five standard mesh cages containing an equal number of 20 (10 x 2) juvenile pearl mussels per stock in separate chambers were installed. Survival and growth rates of juveniles were checked after three months (i.e. before their first winter) and after nine months (i.e. after their first winter). Mussel stock and study stream conditions significantly influenced juvenile performance. Growth rates were determined by study stream conditions and increased with stream water temperature, organic carbon and C/N ratios.Survival rates varied stock-specifically, indicating different levels of local adaptation to their native streams. Due to the detection of stream-specific differences in juvenile performance, freshwater pearl mussels appear suitable as bioindicators. However, a careful of consideration of stock-specificity is necessary to avoid false interpretation of bioindication results. The comparison of stock-specific survival in native versus non-native streams implicates that exposure of juveniles outside their native habitats is able to increase breeding success or else serve for risk spreading in breeding programs.

ABSTRACT1. The lacustrine brown trout (Salmo trutta) is endangered and of high conservation importance. In the only spawning habitat of the population in the Bavarian Lake Walchensee, the River Obernach, a substantial decrease in spawning runs has been reported. In this study, the present ecological state of the spawning stream was analysed with the objective of identifying life-stage specific limitations to successful recruitment attributable to deficiencies in (i) spawning migration, (ii) spawning habitat quality, and (iii) habitat quality for juveniles.2. Structural stream analysis showed that discharge and several migration barriers -particularly near the river outlet into the lake -prevent successful spawning migrations at normal water levels. Migration barriers are probably the main limiting factor for reproduction of lacustrine brown trout, whereas structural variability of the Obernach meets the habitat requirements of both spawners and juveniles.3. Spawning site quality was suitable for trout, as indicated by stream substratum texture and high exchange rates between free-flowing water and the interstitial zone in physico-chemical parameters (redox potential, dissolved oxygen, pH, temperature and conductivity).4. Analyses of fish community structure revealed dominance of lithophilic species, in particular of riverine brown trout (Salmo trutta). Its density and intact demographic population structure suggest that spawning and juvenile habitat quality for salmonids is not limiting. Recapture of stocked lacustrine trout juveniles also indicates habitat suitability for the juvenile stage.5. In conclusion, the results show that the methodology used in this study is suitable for the identification of life-stage specific habitat deficiencies in lacustrine brown trout and other fish species. Availability of habitat data throughout the species' distribution range is a first crucial step for the development of an effective recovery plan.