Background and aims: Oxidative stress is presumed to play an important role in Crohn's disease (CD) pathogenesis. Nevertheless, the evaluation of the intestinal antioxidant capacity through the analysis of glutathione peroxidase activity in CD remains to be determined. Methods: 20 CD outpatients and 16 volunteers going through colonic cancer screening were enrolled. Colonoscopy with biopsies was performed in all individuals. Samples from inflamed and non-inflamed mucosa were taken when there was CD endoscopic activity. Spectrophotometric assays were performed to measure tissue glutathione peroxidase (GPx) activity, and total (GSH T ) and oxidized (GSSG) glutathione in all samples. Demographics and clinical characteristics were collected from clinical charts. Results: Inflamed CD mucosa presented reduced GPx activity compared to non-inflamed CD mucosa (42.94 mU/mg protein vs 79.62 mU/mg protein, P b 0.05) and control mucosa (42.94 mU/mg protein vs 95.08 mU/mg protein, P b 0.001). GSH T concentration was reduced in inflamed mucosa when compared to non-inflamed CD mucosa (0.78 μmol/g vs 1.98 μmol/g, P b 0.01) and the control group (0.78 μmol/g vs 2.11 μmol/g, P b 0.001). A significant correlation was detected between GPx activity and GSSG (r = −0.599), disease duration (r = 0.546), and thiopurine treatment (r = −0.480) in non-inflamed CD mucosa. A v a i l a b l e o n l i n e a t w w w . s c i e n c e d i r e c t . c o m
ScienceDirectJournal of Crohn's and Colitis (2013) 7, e358-e366Downloaded from https://academic.oup.com/ecco-jcc/article-abstract/7/9/e358/426937 by guest on 07 June 2019Conclusion: Our findings suggest that reduced GPx activity is present in inflamed CD mucosa. In addition, endoscopic activity, disease duration and thiopurine therapy could be associated with mucosal decreased antioxidant activity.
This work aimed to evaluate the essential oil yield and composition of the species Myrciaria delicatula, Campomanesia xantocarpha, Campomanesia aurea, Calyptranthes clusiifolia, Myrcia splendens, Eugenia osoriana, Myrciaria tenella, Myrceugenia reitzii, Calyptranthes concinna e Myrcia arborensis from a segment of the Atlantic Forest of Parana State. The sample collections for essential oil extraction, photographic records and herbarium specimen’s preparation for botanical species identification were made at the Private Reserve of Natural Heritage (PRNP) Butuguara in Palmeira (PR), with “Campos Gerais” formations. Myrceugenia reitzii showed eesential oil yield superior to other species and the essential oil yield of all species increased after drying. The chemical composition in the samples showed mono and sesquiterpenes, being the sesquiterpenes in higher percentages. The drying conditions affected the essential oil composition of the for the majority of the evaluated species
The Myrtaceae family has great occurrence in Brazil, mainly in the Atlantic Forest of the South and Southeast regions and presents potential for essential oil production. This work aimed to evaluate the essential oil yield and composition of the species Myrciaria delicatula, Campomanesia xantocarpha, Campomanesia aurea, Calyptranthes clusiifolia, Myrcia splendens, Eugenia osoriana, Myrciaria tenella, Myrceugenia reitzii, Calyptranthes concinna e Myrcia arborensis from a segment of the Atlantic Forest of Parana State. The essential oil extraction was carried out by hydrodistillation of fresh and dried leaves using a Clevenger type apparatus and the chemical composition was analyzed by gas phase chromatography coupled with mass spectrometry (GC/MS). Myrceugenia reitzii showed eesential oil yield superior to other species and the essential oil yield of all species increased after drying. The chemical composition in the samples showed mono and sesquiterpenes , being the sesquiterpenes in higher percentages. The drying conditions affected the essential oil composition of the for the majority of the evaluated species.
SEMINARSto 12.22%. The average stem rot mortality was comparatively low (16-20%) in summer crop but increased 18 to 26% in winter crop. The percentages of root and stem rot mortality in plantation of were initially low but had increased to 37.35% (summer crop) and 26% (winter crop) at Ranichauri.
Sm08.251The ex situ Comparison of Two Improved St. John's Wort (Hypericum perforatum L.) Cultivars with an Iranian Wild PopulationIn this research, two improved cultivars of Hypericum perforatum ('Gold' and 'Veperikon') were compared with a wild Iranian population (Ardabile population) under common garden conditions in Iran. Plants were cultivated from seed in a greenhouse and seedlings were transplanted after one month to the field plots. The statistical design of this study was a Randomized Complete Block Design with three replications. During the period of full flowering, selected phenological (number of days to flowering), morphological (plant height, mean leaf area, number of black nodules/leaf) and chemical (hypericin and pseudohypericin content) characteristics were assessed. Our observations were that the 'Veperikon' cultivar is very sensitive to soil-borne diseases. All transplanted seedlings were infected by the plant pathogenic fungus Colletotrichum gloeosporioides (Penz.), which caused necrosis of the whole plant. Both the 'Gold' cultivar and plants from the wild population persisted despite mild infections with C. gloeosporioides and produced flowering shoots at both the first and second years after cultivation. The 'Gold' cultivar was superior to the Ardabile population in terms of phenological and morphological characteristics. The average naphthodianthrone contents (% dry weight of tissue) for the wild Iranian population were 0.09(±0.03)% but for the 'Gold' cultivar, 0.65(±0.12)%. These data indicate that selection and directed cultivation of Iranian H. perforatum plants can result in plants with improved morphological, phenological and chemical characteristics.
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