Introduction: Biodentine (BD), a dentin substitute, is currently used to treat external cervical root resorption, but its effects on gingival fibroblasts (GFs) are not fully known. Objective: To investigate and compare BD and MTA (mineral trioxide aggre-gate) in terms of proliferative, migratory, and adhesion effects on human pulpal and gingival cells. Material and Methods: Cells were incubated directly on the surface of BD and MTA disks. Adhesion (4 and 24 h) and proliferation (3, 5, 7, 14, 21) were evaluated with crystal violet and MTT assays (n=9 X each group). A wound-healing assay was performed for cell migration, with 0.2 and 2 µg/ml MTA or BD (n=6 X each group). The cut-off point for statistical significance was set at p<0.05, p<0.01 and p<0.001. Results: The best adhesion and proliferation results for gingival fibroblast (GFs) were obtained with BD (p<0.01). MTA and BD enhanced the migration of GFs in a dose-dependent manner, with superior results with BD, and 2 µg/ml was the optimal concentration for enhancing the migration of GFs. Conclusion: Results indicate that BD and MTA exhibit excellent compatibility in terms of cell adhesion, proliferation, and cellular migration. Also, the results suggested that BD is associated with better results than MTA in GFs. The results support the clinical application of BD in areas colonized with GFs.
Bone defects lead to the structural loss of normal architecture, and those in the field of bone tissue engineering are searching for new alternatives to aid bone regeneration. Dental pulp-mesenchymal stem cells (DP-MSC) could provide a promising alternative to repair bone defects, principally due to their multipotency and capacity to fabricate three-dimensional (3D) spheroids. The present study aimed to characterize the 3D DP-MSC microsphere and the osteogenic differentiation capacity potential cultured by a magnetic levitation system. To achieve this, the 3D DP-MSC microsphere was grown for 7, 14, and 21 days in an osteoinductive medium and compared to 3D human fetal osteoblast (hFOB) microspheres by examining the morphology, proliferation, osteogenesis, and colonization onto PLA fiber spun membrane. Our results showed good cell viability for both 3D microspheres with an average diameter of 350 μm. The osteogenesis examination of the 3D DP-MSC microsphere revealed the lineage commitment, such as the hFOB microsphere, as evidenced by ALP activity, the calcium content, and the expression of osteoblastic markers. Finally, the evaluation of the surface colonization exhibited similar patterns of cell-spreading over the fibrillar membrane. Our study demonstrated the feasibility of forming a 3D DP-MSC microsphere structure and the cell-behavior response as a strategy for the applications of bone tissue guiding.
The present study aimed to compare the adhesion and proliferation of human periodontal ligament fibroblasts (hPDL) in transverse sections of the teeth sealed with two different obturation techniques, BioRoot RCS/hydraulic obturation (HO) and AH-Plus/continuous-wave condensation (CWC). The techniques were tested using an in vitro model to simulate the interaction between periodontal tissues and the materials. The root canals were instrumented and sterilized. A total of 15 samples were obturated with BioRoot RCS/HO and 15 samples with AH-Plus/CWC. Then, roots were sectioned to obtain obturated teeth slices, and hPDL cells were seeded onto the root slices. The results were obtained at intervals of 4 and 24 h for cell adhesion; and at 3,7,14, and 21 days for cell proliferation. Empty cell culture plates were use as controls. The cell adhesion was increased at 4 and 24 h for both groups, with an increased response observed in the BioRoot RCS/HO group (p<0.05). The difference in cell proliferation was also found between experimental groups. After 14 days of culture, BioRoot RCS/HO group showed an increase response than control and AH-Plus/CWC groups (p<0.05), and after 21 days both groups behaved better than control group, with an increased response observed in the BioRoot RCS/HO group. This study demonstrated that both root canal sealers allow the attach and growth of periodontal ligament fibroblasts, with an increased biological response in the BioRoot RCS/HO group.
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