We present the first records of Myotis nigricans (Schinz, 1821) for Santa Fe province, Argentina. Four specimens were collected in the following localities: Esperanza (Las Colonias department), Laguna Paiva (La Capital department), and Rosario (Rosario department). These records extend the distribution area of M. nigricans 380 km to the south and increase the number of bat species in Santa Fe to 23. Additionally, Esperanza and Rafaela are added as new localities for Eumops glaucinus (Wagner, 1843), Molossops temminckii (Burmeister, 1854), and Lasiurus blossevillii (Lesson & Garnot, 1826).
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