Highlights
Blood flow restriction at resting periods of high-intensity load exercise increases Pax7 expression.
miR-206 levels significantly decreased in the blood flow restriction leg compared to the control.
Blood flow restriction can cause DNA damage, judging from the increase in messenger RNA levels of Ku70.
The aim of the study was to develop and assess the reliability of a functional agility test containing offensive elements for water polo players. Eighteen young male (15.3 ± 0.5 years, 178.3 ± 4.7 cm, 69.4 ± 10.0 kg) water polo players with a minimum of 5 years of sport-specific experience participated in this study. The test contained reactive high-intensity short-term swimming with changes in direction and manoeuvres after perceiving unknown stimuli given by tester players, and also included a shooting task at a goal, first from 7 m and then from 5 m. Execution time and shooting efficiency were measured by two experienced water polo coaches (Evaluators A and B). All statistical analyses were calculated using SPSS. The intrarater reliability between attempts showed good reliability for both evaluators (Evaluator A: ICC: 0.87; 95% CI: 0.66–0.95 and Evaluator B: ICC: 0.88; 95% CI: 0.68–0.96). Interrater reliability between Evaluators A and B was excellent at both attempts (Attempt 1: ICC: 0.97; 95% CI: 0.93–0.99 and Attempt 2: ICC: 0.98; 95% CI: 0.91–0.99). A lack of correlation between shooting performance from 7 m and 5 m distances and execution time was observed in the protocol. The test we presented in this study was found to be a reliable measurement tool for testing offensive agility performance based on open skill nature among water polo players.
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