We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = − 0.102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica.
We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Nontoxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = -0.102) were both low. Clustering was not related to the geographical origin of accessions.International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. 24 We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican 25 germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the 26 phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was 27 evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum 28 Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively.
We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Nontoxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = -0.102) were both low. Clustering was not related to the geographical origin of accessions.International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. 24 We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican 25 germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the 26 phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was 27 evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum 28 Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively.
We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Nontoxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = -0.102) were both low. Clustering was not related to the geographical origin of accessions.International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. 24 We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican 25 germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the 26 phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was 27 evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum 28 Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.