A study was conducted in a greenhouse, to investigate the effects of arbuscular mycorrhizal fungi (Glomus intraradices), soil salinity and P availability on growth (leaf area and dry weight), nutrient absorption and ion leakage, chlorophyll, soluble sugar and proline content and alkaline phosphatase activity of pepper plants (Capsicum annuum L.). Plants were grown at four levels of salinity (0, 50, 100 and 200 mM NaCl) and two P levels (10 and 40 mg kg -1). Colonisation was 80% to 51% in non-stressed and high salt-stressed plants, respectively. The mycorrhizal dependency was high and only reduced at the higher salinity level. Mycorrhizal plants maintained greater root and shoot biomass at all salinity levels compared to non-mycorrhizal plants, regardless the P level. Interactions between salinity, phosphorous and mycorrhizae were significant for leaf area, root and shoot dry mass. Non-mycorrhizal plants accumulated higher Na and lower K and P compared to mycorrhizal plants. The cell membrane integrity was greater in mycorrhizal plants than in non-mycorrhizal ones. The proline content increases with increasing salt stress and was significantly higher in leaves than in roots The results indicate that the mycorrhizal inoculation is capable of alleviating the damage caused by salt stress conditions on pepper plants, to maintaining the membranes stability and plant growth, and this could be related to P nutrition.

In general, heavy metals interfere with several physiological processes and reduce plant growth. Plants naturally establish symbiotic associations with soil microorganisms, such as mycorrhizal fungi. The aim of this research was to determine if inoculation with mycorrhizal fungi increases tolerance to Cr, evidenced by growth and biochemical parameters and the effect on roots membranes in Capsicum annum. Plants were either non-inoculated or inoculated with Glomus mosseae or Glomus intraradices, and grown in the presence of different concentration of Cr (K 2 Cr 2 O 4 ) in soil. Pepper plants grown without Cr behaved as mycotrophic species. At the highest concentration (200 mM K 2 Cr 2 O 4 ), Cr reduced root colonization by G. mosseae or G. intraradices (to 23 and 20% respectively). Moderate and high concentrations of Cr reduced all growth parameters. The interaction of inoculation and Cr increased leaf chlorophyll and proline content while reduced the leaf protein and root proline content. Carotenoid content was not affected by treatments. High Cr concentrations increased significantly electrolytes leakage in roots, either non-inoculated or inoculated plants. At the highest Cr concentration, inoculated plants had double the biomass of non-inoculated plants. Cr content in roots of inoculated plants was significantly higher than in non-inoculated plants. Chromium accumulation was low in leaves and showed no differences between treatments. Mycorrhization increased pepper plant tolerance to Cr in the soil, modifying proline metabolism to assure a more efficient response.

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RESUMO: Inoculação com fungos micorrízicos e adubação fosfatada no crescimento, produção de óleo essencial e absorção de nutrientes em hortelã-pimenta (Mentha piperita L.). Este estudo avaliou os efeitos da inoculação de fungos micorrízicos arbusculares Glomus mosseae, Glomus intraradices A 4 e Glomus intraradices B 1 e duas doses de fósforo (10 e 40 mg kg -1 ) sobre a colonização radicular, crescimento, absorção de nutrientes e óleos essenciais em Mentha piperita L. O estudo foi conduzido em casa de vegetação no delineamento inteiramente casualizado em esquema fatorial 4x2. Sessenta dias após o transplantio, as plantas micorrizadas apresentaram massa fresca, massa seca, e área foliar significativamente maior em comparação as não-micorrizadas. A inoculação aumentou o teor de P, K e Ca na parte aérea sendo superiores em 40 mg P kg -1 de solo. As plantas cultivadas com 40 mg P kg -1 de solo aumentaram a produção de óleo essencial por planta cerca de 40-50% em relação às cultivadas com 10 mg de P kg -1 , independentemente da micorrização. Dentre as espécies fúngicas estudadas, a inoculação com G. Intraradices A 4 e com um elevado nível de P, aumentou significativamente o crescimento e rendimento de óleos essenciais em comparação com outras espécies de fungos micorrízicos estudados. Em conclusão, a inoculação dos fungos micorrízicos arbusculares em plantas de hortelã é uma alternativa viável para aumentar a produção de óleos essenciais e reduzir o uso de fertilizantes necessários para a produção econômica de hortelã-pimenta com deficiência de fósforo no solo. Palavras-chave: adubação fosfatada, Glomus mosseae, Glomus intraradices, Mentha piperita, óleo essencialABSTRACT: This study evaluated the effects of inoculation with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices A 4 and Glomus intraradices B 1 and two phosphorus levels (10 and 40 mg kg -1 ) on root colonization, plant growth, nutrient uptake and essential oil content in Mentha piperita L. The experiment was carried out in a greenhouse, in 4x2 factorial arrangement, in completely randomized design. At sixty days after transplanting, the mycorrhizal plants had significantly higher fresh matter, dry matter and leaf area compared to non-mycorrhizal plants. The inoculation increased P, K and Ca levels in the shoot which were higher under 40 mg P kg -1 of soil. Plants grown with 40 mg P kg -1 soil increased the essential oil yield per plant by about 40-50% compared to those cultivated with 10 mg P kg -1 , regardless of the mycorrhizal treatment. Among the studied fungal species, inoculation with G. intraradices A 4 and a high level of P significantly increased plant growth and essential oil yield, compared to the other studied mycorrhizal fungal species. In conclusion, inoculation of arbuscular mycorrhizal fungi into peppermint plants is a feasible alternative to increase the essential oil production and reduce the use of fertilizers required to obtain economic production of peppermint under phosphorus-deficient soil condition.

The effect of arbuscular mycorrhizal fungi (AMF) inoculation, on pepper plant growth and physiological parameters in response to increasing soil Cu concentrations was studied. Treatments consisted of inoculation or not with Funneliformis mosseae or Rhizophagus intraradices and the addition of Cu to soil at concentrations of 0, 2, 4 and 8 mM CuSO 4 . The increase in copper concentration diminished the inoculation in all treatments. The highest experimental concentration of Cu (8 mM) reduced significantly the hyphae viability and ALP activity, regardless of the inocula used. The total dry weight and the leaf area were higher for mycorrhizal plants. The mycorrhizal dependence was 30 and 50% for plants inoculated with F. mosseae and R. intraradices, respectively at 8 mM CuSO 4 . The electrolyte leakage was higher at higher Cu concentrations, in roots and leaves. Net photosynthetic rates and transpiration were lower in plants treated with Cu, regardless of the inocula. At low Cu concentration in soil no differences were observed in Cu content in the shoots and roots. At 4 mM Cu, Cu content in roots was significantly higher than in shoots. At 8 mM Cu, in non-inoculated plants accumulate more Cu than inoculated plants in the roots, leaves and fruits regardless of the inocula. Beneficial microbial inoculants such as AMF, is an attractive strategy to farmers in the context of sustainable agriculture. Pre-inoculation in transplants could be an adequate practice to alleviate the deleterious effects in stress of pepper plants. However different AMF species can differ in their ability to minimize stress effects and promote plant growth.

When glyphosate is added to the soil, it is absorbed by roots and transported by xylem causing growth inhibition in plants. Mycorrhiza is the beneficial association between roots of most plants and soil fungi. The methylphosphonic group of the glyphosatecouldcompetewithinorganicphosphatesforsorptionsitesinthesoil.Theaimofthisworkwastostudytheeffectof phosphorus availability and glyphosate residues in soil on pepper plant growth, and on physiological parameters, in plants noninoculated or inoculated with Glomus mosseae or G. intraradices.Thephytotoxiceffectsoftheglyphosatewereassessedbyabioindicatorasshikimicacid.Athighdoses,glyphosate(6.32μM)reducedrootcolonization,andthiseffectwasincreasedbyhigher levelsofphosphorusinthesoil.The effectsofherbicideonshikimicacidaccumulationandonshootgrowthbegan24hoursafter glyphosate treatments(HAT).At24,48,and72HAT,inoculatedplantsgrownwithoutglyphosateshowedhighergrowthcompared tothe non-inoculatedones.Athighglyphosate(6.32μM)and96HAT,thegrowthwascompletelyinhibited.Theshikimicacidaccumulatedintheupperleavesofnon-inoculatedplants,treatedat3.16μMglyphosate,wassignificantlyhigherathighPlevel,related toinoculatedones.Theseresultssuggestthattheremobilizationofglyphosateresiduesinthesoilbytheadditionofphosphate shouldbeconsideredaseriousproblemforcropsintreatedsoils.Themycorrhizationincreasesthepepperplant'stolerancetohigh glyphosate concentration in the substrate, and may allow support to this stress condition.

[D-Leu1]MC-LR and MC-LR, two microcystins differing in one amino acid, constitute a sanitary and environmental problem owing to their frequent and concomitant presence in water bodies of the Americas and their association with human intoxication during recreational exposure to cyanobacterial bloom. Present in reservoirs used for irrigation as well, they can generate problems in the development of crops such as Phaseolus vulgaris, of nutritional and economic interest to the region. Although numerous works address the toxic effects of MC-LR, information on the toxicity of [D-Leu1]MC-LR is limited. Our objective was to study the toxic effects of [D-Leu1]MC-LR and MC-LR (3.5 µg/ml) on P. vulgaris after a single contact at the imbibition stage. Our findings indicate that 10 days post treatment, [D-Leu1]MC-LR generates morphological and physiological alterations more pronounced than those caused by MC-LR. In addition to the alterations produced by [D-Leu1]MC-LR in the development of seedlings and the structure of the leaves, roots and stems, we also found alterations in leaf stomatal density and conductivity, a longer delay in the phototropic response and a decrease in the maximum curvature angles achieved with respect to that observed for MC-LR. Our findings indicate that these alterations are linked to the greater inhibition of phosphatase activity generated by [D-Leu1]MC-LR, rather than to oxidative damage. We observed that 30 days after treatment with MC-LR, plants presented better development and recovery than those treated with [D-Leu1]MC-LR. Further studies are required on [D-Leu1]MC-LR and MC-LR toxicity and their underlying mechanisms of action.

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