A osteonecrose dos maxilares induzida por bisfosfonatos (OMB) é um efeito adverso grave decorrente do tratamento com Ácido Zoledrônico (AZ) e está associada a desregulação imune-inflamatória. A curcumina é um inibidor da oligomerização deNFkB podendo constituir importante ferramenta como terapia adjuvante. O objetivo desse estudo é avaliar a influência da curcumina na severidade da OMB. Foram utilizados 50 ratos Wistar machos divididos em um grupo controle tratado comsolução salina, um grupo tratado com AZ (0,20mg/kg) e três grupos testes co- tratados com curcumina 75, 150 ou 300 mg/kg (GTC-75, -150, -300). Os animais foram submetidos a quatro administrações venosas de AZ e exodontia do primeiro molarinferior esquerdo. Solução salina e os fármacos foram administrados por gavagem desde o início do protocolo nas doses supracitadas três vezes por semana até o fim do protocolo (dia 70). As mandíbulas foram submetidas a análise radiográfica ehistomorfométrica (contagem de lacunas de osteócitos vazias, osteoclastos normais e apoptóticos e células inflamatórias polimorfonucleares (PMN) e mononucleares (MN). Teste ANOVA/Bonferroni foi utilizado (p<0,05). Após tratamento comcurcumina houve redução da área radiolúcida sugestiva de OMB (p<0,001), percentual de lacunas de osteócitos vazias (p<0,001) osteoclastos (p<0,001) e células inflamatórias PMN (p<0,001) e MN (p<0,001).Conclusão: A curcumina melhorou o reparo alveolar e preveniu lesões osteonecroticas pós exodontia.
Purpose: To evaluate the effect of a selective cyclooxygenase 2 (COX-2) inhibitor on trigeminal ganglion changes and orofacial discomfort/nociception in rats submitted to an experimental model of dental occlusal interference (DOI). Methods: Female Wistar rats (180-200 g) were divided into five groups: a sham group (without DOI) (n=15); and four experimental groups with DOI treated daily with 0.1 mL/kg saline (DOI+SAL), 8, 16, or 32 mg/kg celecoxib (DOI+cel -8, -16, -32) (n=30/group). The animals were euthanized after one, three, and seven days. The bilateral trigeminal ganglia were analyzed histomorphometrically (neuron cell body area) and immunohistochemically (COX-2, nuclear factor-kappa B [NFkB], and peroxisome proliferator-activated receptor-y [PPARy]). A bilateral nociception assay of the masseter muscle was performed. The number of bites/scratches, weight, and grimace scale scores were determined daily. One-way/two-way analysis of variance (ANOVA)/Bonferroni post hoc tests were used (P < .05, GraphPad Prism 5.0). Results: DOI+SAL showed a reduction in neuron cell body area bilaterally, whereas DOI+cel-32 exhibited a significative increase in neuron cell body area compared with DOI+SAL group (P < 0.05). The ipsilateral (P=0.007 and P=0.039) and contralateral (P < 0.001 and P=0.005) overexpression of COX-2 and NFkB and downregulation of PPARy (P=0.016 and P < 0.001) occurred in DOI+SAL, but DOI+cel-32 reverted this alteration. DOI+SAL showed increase in isplateral (P < 0.001) and contralateral (P < 0.001) nociception, an increased number of bites (P=0.010), scratches (P < 0.001), and grimace scores (P=0.032). In the group of DOI+cel-32, these parameters were reduced. Conclusions: Celecoxib attenuated DOI-induced transitory nociception/orofacial discomfort resulting from trigeminal COX-2 overexpression.
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