Summary1. Tropical forests are globally important, but it is not clear whether biodiversity enhances carbon storage and sequestration in them. We tested this relationship focusing on components of functional trait biodiversity as predictors. 2. Data are presented for three rain forests in Bolivia, Brazil and Costa Rica. Initial above-ground biomass and biomass increments of survivors, recruits and survivors + recruits (total) were estimated for trees ≥10 cm d.b.h. in 62 and 21 1.0-ha plots, respectively. We determined relationships of biomass increments to initial standing biomass (AGB i ), biomass-weighted community mean values (CWM) of eight functional traits and four functional trait variety indices (functional richness, functional evenness, functional diversity and functional dispersion). 3. The forest continuum sampled ranged from 'slow' stands dominated by trees with tough tissues and high AGB i , to 'fast' stands dominated by trees with soft, nutrient-rich leaves, lighter woods and lower AGB i . 4. We tested whether AGB i and biomass increments were related to the CWM trait values of the dominant species in the system (the biomass ratio hypothesis), to the variety of functional trait values (the niche complementarity hypothesis), or in the case of biomass increments, simply to initial standing biomass (the green soup hypothesis). 5. CWMs were reasonable bivariate predictors of AGB i and biomass increments, with CWM specific leaf area SLA, CWM leaf nitrogen content, CWM force to tear the leaf, CWM maximum adult height H max and CWM wood specific gravity the most important. AGB i was also a reasonable predictor of the three measures of biomass increment. In best-fit multiple regression models, CWM H max was the most important predictor of initial standing biomass AGB i . Only leaf traits were selected in the best models for biomass increment; CWM SLA was the most important predictor, with the expected positive relationship. There were no relationships of functional variety indices to biomass increments, and AGB i was the only predictor for biomass increments from recruits. 6. Synthesis. We found no support for the niche complementarity hypothesis and support for the green soup hypothesis only for biomass increments of recruits. We have strong support for the biomass ratio hypothesis. CWM H max is a strong driver of ecosystem biomass and carbon storage and CWM SLA, and other CWM leaf traits are especially important for biomass increments and carbon sequestration.

Question How reliable is the process of delimiting plant species by morphotyping sterile specimens from a highly diverse Amazonian forest plot? Location Biological Dynamics of Forest Fragments Project (BDFFP), Central Amazon, Manaus, Brazil. Methods A taxonomic exercise was conducted during a Center for Tropical Forest Science (CTFS) Taxonomy Workshop held in Manaus in April 2011, using specimens collected in a 25‐ha forest plot. The plant species from this plot had been previously delimited by morphotyping of ca. 80 000 sterile specimens, a process that resulted in the recognition of 115 cases (accounting for 38% of all trees) in which species delimitation was problematic. For the workshop, we selected a subsample of specimens for eight of these difficult cases (taxonomic groups/complexes) and asked 14 participants with different levels of botanical training to independently sort these specimens into morphospecies. We then compared the classifications made by all participants and explored correlations between botanical training and plant classification. Results The classification of specimens into morphospecies was highly variable among participants, except for one taxonomic group/complex, for which the median pair‐wise similarity was 95%. For the other seven taxonomic groups/complexes, median pair‐wise similarity values ranged from 52% to 67%. Training did not increase the similarity in the definition of morphospecies except for two taxonomic groups/complexes, for which there was higher congruence between the classifications made by participants with a high level of botanical training than in comparisons that included less‐experienced participants. The total number of morphospecies defined by participants was highly variable for all taxonomic groups/complexes, with the total number varying from 12 to 46 (a 383% difference). Conclusions Local plant species delimitation by morphotyping sterile specimens is prone to large uncertainties, and botanical training may not reduce them. We argue that uncertainty in species delimitation should be explicitly considered in plant biodiversity inventories as diversity estimates may be strongly affected by such uncertainties. We recommend that species delimitation and identification be treated as separate processes and that difficulties be explicitly recorded, so as to permit error estimates and the refinement of taxonomic data.

Disentangling the mechanisms that shape community assembly across diversity gradients is a central matter in ecology. While many studies have explored community assembly through species average trait values, there is a growing understanding that intraspecific trait variation (ITV) can also play a critical role in species coexistence. Classic biodiversity theory hypothesizes that higher diversity at species-rich sites can arise from narrower niches relative to species-poor sites, which would be reflected in reduced ITV as species richness increases. To explore how ITV in woody plant communities changes with species richness, we compiled leaf trait data (leaf size and specific leaf area) in a total of 521 woody plant species from 21 forest communities that differed dramatically in species richness, ranging from boreal to tropical rainforests. At each forest, we assessed ITV as an estimate of species niche breadth and we quantified the degree of trait overlap among co-occurring species as a measure of species functional similarity. We found ITV was relatively invariant across the species richness gradient. In addition, we found that species functional similarity increased with diversity. Contrary to the expectation from classic biodiversity theory, our results rather suggest that neutral processes or equalizing mechanisms can be acting as potential drivers shaping community assembly in hyperdiverse forests.

Abstract:The functional composition of plant communities in montane regions has been studied for decades, and most recent analyses find that environmentally favourable landscapes at lower altitudes tend to be dominated by species with resource-acquisitive traits, while more resource-conservative taxa dominate higher-altitude communities. However, it is unclear the extent to which this pattern is driven by co-gradient variation within clades or changes in clade representation across the gradient. To test for co-gradient variation, species composition, phylogenetic structure and functional traits were quantified for 97 species within the plant family Melastomataceae at five locations across a 2500-m altitudinal gradient along Volcán Barva in Costa Rica. Average melastome leaf force to punch, specific leaf area and leaf size vary with altitude, while four other functional traits do not. Taxonomic dissimilarity between communities was correlated with altitudinal difference, while phylogenetic dissimilarity was correlated with altitudinal dissimilarity only when measured with a metric that emphasizes shallow turnover of the tips of the phylogeny. These results highlight how species turnover may be more pronounced than functional or phylogenetic variation along altitudinal gradients. In addition, these results highlight the conservation value of lowland tropical forests, which here harbour a disproportionate amount of phylogenetic and functional diversity.